Human MIP-1 beta (CCL4) Recombinant

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Human MIP-1 beta (CCL4) Recombinant


accession P13236

Source Optimized DNA sequence encoding Human CCL4/MIP-1 beta chain was expressed in Escherichia Coli.
Molecular weight Native human MIP-1 beta is generated by the proteolytic removal of the signal peptide and propeptide. This molecule has a calculated molecular mass of approximately 8 kDa. Recombinant CCL4/MIP-1 beta is a disulfide-linked homodimeric protein consisting of 69 amino acid residue subunits,and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity Determined by its ability to chemoattract human blood monocytes using a concentration range of.0-10.0 ng/ml.

Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant MIP-1 beta was lyophilized from a.2 μm filtered PBS solution.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Interactor P51677
Interactor P32246
Interactor P32302
Interactor P51681
Biological Process Chemotaxis
Biological Process Inflammatory-response
Molecular function Cytokine


ELISA confirms CBMC antiviral chemokine release following antibody-enhanced dengue virus infection or polyI:C exposure.

  • After 24 hours, cell supernatants were harvested and measured for CCL4, CCL5 and CXCL10 by ELISA.

(A) Relative quantification of chemokine mRNA expression to GUS and IPO8 by tissue type.

20 ng ml−1 recombinant CCL4
  • Western blots probing for CCL3, CCL4 and GUS in eight samples of CRC with matched distal colon of standard protein concentration (2 mg BSA per ml).