Human MIP-1 beta (CCL4) Recombinant
$70.00 – $2,700.00
| accession | P13236 |
| Source | Optimized DNA sequence encoding Human CCL4/MIP-1 beta chain was expressed in Escherichia Coli. |
| Molecular weight | Native human MIP-1 beta is generated by the proteolytic removal of the signal peptide and propeptide. This molecule has a calculated molecular mass of approximately 8 kDa. Recombinant CCL4/MIP-1 beta is a disulfide-linked homodimeric protein consisting of 69 amino acid residue subunits,and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Biological Activity | Determined by its ability to chemoattract human blood monocytes using a concentration range of.0-10.0 ng/ml. |
| Protein Sequence | MKLCVTVLSL LMLVAAFCSP ALSAPMGSDP PTACCFSYTA RKLPRNFVVD YYETSSLCSQ PAVVFQTKRS KQVCADPSES WVQEYVYDLE LN |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
| Presentation | Recombinant MIP-1 beta was lyophilized from a.2 μm filtered PBS solution. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers |
| Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | P51677 |
| Interactor | P32246 |
| Interactor | P32302 |
| Interactor | P51681 |
| Biological Process | Chemotaxis |
| Biological Process | Inflammatory-response |
| Molecular function | Cytokine |
Methods
ELISA confirms CBMC antiviral chemokine release following antibody-enhanced dengue virus infection or polyI:C exposure.
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(A) Relative quantification of chemokine mRNA expression to GUS and IPO8 by tissue type.
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