Human MIP-1 alpha (CCL3) Recombinant ,Biotinylated
$70.00 – $2,700.00
| accession | P10147 |
| Source | DNA sequence encoding Human MIP-1A mature chain was expressed in Escherichia Coli. MIP1A was further labeled by Biotin for detection by Avidin conjugated secondary probes |
| Molecular weight | Native human MIP-1 alpha is generated by the proteolytic removal of the signal peptide.This molecule has a calculated molecular mass of approximately 8 kDa. Recombinant MIP-1 alpha is a monomer protein consisting of 70 amino acid residue subunits, andmigrates as an approximately8 kDa protein under non-reducing andreducing conditions in SDS-PAGE. |
| Purity | >98%, as determined by SDS-PAGE and HPLC |
| Applications | Biotinylated MIP-1A was designed to be used in single step detection in ELISA ,WB |
| Protein Sequence | MQVSTAALAV LLCTMALCNQ FSASLAADTP TACCFSYTSR QIPQNFIADY FETSSQCSKP GVIFLTKRSR QVCADPSEEW VQKYVSDLEL SA |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
| Presentation | Recombinant MIP-1 was lyophilized from a.2 μm filtered PBS solution pH7.5. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | P32302 |
| Interactor | P32246 |
| Interactor | P51677 |
| Interactor | P51681 |
| Biological Process | Chemotaxis |
| Biological Process | Inflammatory-response |
| Molecular function | Cytokine |