Human MIP-1 alpha (CCL3) Recombinant ,Biotinylated

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Human MIP-1 alpha (CCL3) Recombinant ,Biotinylated


accession P10147

Source DNA sequence encoding Human MIP-1A mature chain was expressed in Escherichia Coli. MIP1A was further labeled by Biotin for detection by Avidin conjugated secondary probes
Molecular weight Native human MIP-1 alpha is generated by the proteolytic removal of the signal peptide.This molecule has a calculated molecular mass of approximately 8 kDa. Recombinant MIP-1 alpha is a monomer protein consisting of 70 amino acid residue subunits, andmigrates as an approximately8 kDa protein under non-reducing andreducing conditions in SDS-PAGE.
Purity >98%, as determined by SDS-PAGE and HPLC
Applications Biotinylated MIP-1A was designed to be used in single step detection in ELISA ,WB

Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant MIP-1 was lyophilized from a.2 μm filtered PBS solution pH7.5.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Interactor P32302
Interactor P32246
Interactor P51677
Interactor P51681
Biological Process Chemotaxis
Biological Process Inflammatory-response
Molecular function Cytokine