Human MCP1 Recombinant, Biotinylated
Categories: Biotinylated proteinsCXC chemokines$160.00 – $3,500.00
Description
Accession
P13500
Source
DNA sequence encoding Human MCP-1 mature chain was expressed in Escherichia Coli. MCP1 was further labeled by Biotin for detection by Avidin conjugated secondary probes
Molecular weight
Native human MCP1 alpha, generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated molecular mass of approximately 8 kDa. Recombinant MCP-1 alpha is a monomer protein consisting of 76 amino acid residue subunits, migrates as an approximately8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity
>97%, as determined by SDS-PAGE and HPLC
Applications
ELISA,WB Biotinylated SDF-1A was designed to be used in single step detection in ELISA ,WB
Protein Sequence
QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant SDF-1 alpha was lyophilized from a 0.2μm filtered PBS, pH.4.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
P32246
Interactor
P41597
Interactor
P41597
Interactor
P51681
Interactor
Q9NPB9
Biological Process
Biological Process
Molecular function
