Human Macrophage Colony stimulating Factor Recombinant

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Human Macrophage Colony stimulating Factor Recombinant

$70.00$4,700.00


accession P09603


Source Optimized DNA sequence encoding Human M-CSF extracellular domain was expressed in baculovirus infected Bombyx Mori.
Molecular weight Human MCSF, generated by the proteolytic removal of the signal peptide and propeptide, The molecule has a calculated molecular mass of approximately37 kDa. Recombinant Human M-CSF is a disulfide-linked homodimeric protein consisting of twoamino acid residue subunits, andmigrates as an approximately kDa protein under non-reducing and as-21kDa under reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity The ED(50) was determined by the dose-dependent stimulation of the proliferation of of M-NFS-60 cells is <.0 ng/ml, corresponding to a specific activity of > x units/mg.
Protein Sequence MTAPGAAGRC PPTTWLGSLL LLVCLLASRS ITEEVSEYCS HMIGSGHLQS LQRLIDSQME TSCQITFEFV DQEQLKDPVC YLKKAFLLVQ DIMEDTMRFR DNTPNAIAIV QLQELSLRLK SCFTKDYEEH DKACVRTFYE TPLQLLEKVK NVFNETKNLL DKDWNIFSKN CNNSFAECSS Q DVVTKPDCN CLYPKAIPSS DPASVSPHQP LAPSMAPVAG LTWEDSEGTE GSSLLPGEQP LHTVDPGSAK QRPPRSTCQS FEPPETPVVK DSTIGGSPQP RPSVGAFNPG MEDILDSAMG TNWVPEEASG EASEIPVPQG TELSPSRPGG GSMQTEPARP SNFLSASSPL PASAKGQQPA DVTGTALPRV GPVRPTGQDW NHTPQKTDHP SALLRDPPEP GSPRISSLRP QGLSNPSTLS AQPQLSRSHS SGSVLPLGEL EGRRSTRDRR SPAEPEGGPA SEGAARPLPR FNSVPLTDTG HERQSEGSSS PQLQESVFHL LVPSVILVLL AVGGLLFYRW RRRSHQEPQR ADSPLEQPEG SPLTQDDRQV ELPV
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant M-CSFwas lyophilized from a.2 μm filtered PBS solution pH.0 containing% HSA,% mannitol.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor P09581 CSF1R_MOUSE
Interactor P01730 CD4_HUMAN
Interactor P03228
Interactor P07333 CSF1R_HUMAN
Interactor P0CW72
Interactor Q8UZD0
Biological Process Immunity
Biological Process Inflammatory-response
Molecular function Cytokine
Molecular function Growth-factor

Methods

L-Cystine dose-dependently increased TNF-alpha from CD14+ monocytes with LPS under the amino acid environment of patients with advanced cirrhosis.

1,000 U/mL M-CSF
  • A, Isolated CD14+ monocytes (purity >90%) were cultured at a density of 2.5×105 cells/well in 96-well plates containing in ACM and ACM plus L-Cys (L-Cys : 150 nmol/mL) with 1,000 U/mL M-CSF.

Effects of β2-microglobulin (β2m) fibrils on RANKL-dependent osteoclast formation.

750 ng/ml M-CSF
  • Primary human monocytes were incubated with M-CSF and the indicated concentrations of RANKL in the presence of PBS or 10 µg/ml β2m-fibrils for 14 days and their ability to resorb an osteologic substrate was then assessed.

Cell preparation

  • Human peripheral blood was taken from the antecubital vein of healthy volunteers and collected into tubes containing sodium citrate (1% final concentration) to prevent coagulation.
  • Leukocyte populations were then isolated by sedimentation with dextran (500,000 M.wt) followed by discontinuous Percoll™ gradient centrifugation, as reported previously +ve monocytes were cultured for 6 days in tissue culture plates in Iscove's Modified ulbecco's Medium (IMM; ) containing penicillin/streptomycin (PPA , 50 U/ml) and 10% heat-inactivated FCS , in the presence of 50 U/ml GM-CSF to generate Mph1, or 25 ng/ml M-CSF to generate Mph2.
  • Following stimulation with LPS (E.coli, serotype O127∶B8) (10 ng/ml for 24 h) Mph1 cells secreted IL-12 but not IL-10, while Mph2 secreted IL-10 but little IL-12, confirming macrophage polarization

Tks5 as a potential mediator of fusion-competent protrusion formation.

recombinant human M-CSF
  • (E and F) Mouse bone marrow–derived macrophages cultured in the presence of 10 ng/ml M-CSF with or without 10 ng/ml RANKL for the indicated times were subjected either to quantitative RT-PCR analysis of the amount of Sh3pxd2a mRNA (normalized by that of Actb mRNA; E) or to immunoblot analysis with the indicated antibodies .

Tks5 as a potential mediator of fusion-competent protrusion formation.

recombinant human M-CSF
  • (E and F) Mouse bone marrow–derived macrophages cultured in the presence of 10 ng/ml M-CSF with or without 10 ng/ml RANKL for the indicated times were subjected either to quantitative RT-PCR analysis of the amount of Sh3pxd2a mRNA (normalized by that of Actb mRNA; E) or to immunoblot analysis with the indicated antibodies .

Impaired osteoclast differentiation, maturation and functionality in osteoporotic mice received CD34+ cells.

20 ng/ml M-CSF
  • Harvested bone marrow was subjected to differentiation towards osteoclasts using M-CSF and sRANKL (n = 5).

Human macrophage preparations

  • Human macrophages were prepared as previously described 6 cells/ml, and seeded in six-well plates in RPMI 1640 medium supplemented with 10% heat-inactivated FBS .
  • Macrophages were differentiated from monocytes in the presence of recombinant human M-CSF (50 ng/ml, , ) for 6 to 11 days.
  • The purity of the cell preparations was evaluated by flow cytometry and over 85% of CD11b+/CD206+ macrophages were consistently obtained.

Effect of inositol hexakisphosphate (IP6) on human peripheral blood mononuclear cells (PBMNC) osteoclastogenesis and resorption activity on mature osteoclasts cells derived from human PBMNC.

25 ng/ml M-CSF
  • RANKL# refers to cells treated with RANKL and also with M-CSF and dexamethasone as described in the Materials & Methods section.

Effect of inositol hexakisphosphate (IP6) on human peripheral blood mononuclear cells (PBMNC) osteoclastogenesis and resorption activity on mature osteoclasts cells derived from human PBMNC.

25 ng/ml M-CSF
  • RANKL# refers to cells treated with RANKL and also with M-CSF and dexamethasone as described in the Materials & Methods section.

Expression of Rho GTPases on protein level.

M-CSF
  • Some expression is observed in immature DCs (iDC), while prominent expression is observed in macrophages differentiated with GM-CSF or M-CSF and in osteoclasts derived from monocytes (mono) or DC .