Human LIF Recombinant ,Biotinylated

Human LIF Recombinant ,Biotinylated

$70.00$1,700.00


accession P15018


Source DNA sequence encoding Human LIF mature chain [23AA-202AA] fused to polyHis AVI tag was expressed in HEK293 cells.was further labeled by Biotin for detection by Avidin conjugated secondary probes
Molecular weight Native human LIF is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 23kDa. Recombinant LIF is a monomer protein and migrates due to glycosylation as an approximately 40 kDa protein under reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC.
Biological Activity The activity wad determined by the ability to bind recombinant Human LIF receptor in a functional ELISA assay with a linear range of 1-50ng/ml.
Protein Sequence MKVLAAGVVP LLLVLHWKHG AGSPLPITPV NATCAIRHPC HNNLMNQIRS QLAQLNGSAN ALFILYYTAQ GEPFPNNLDK LCGPNVTDFP PFHANGTEKA KLVELYRIVV YLGTSLGNIT RDQKILNPSA LSLHSKLNAT ADILRGLLSN VLCRLCSKYH VGHVDVTYGP DTSGKDVFQK KKLGCQLLGK YKQIIAVLAQ AF
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation RecombinantLeukemia Inhibitory Factorwas lyophilized from a.2 μm filtered PBS pH.5.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor P42702 LIFR_HUMAN
Interactor P42703 LIFR_MOUSE
Molecular function Cytokine
Molecular function Growth-factor