Description
Accession
O14625
Source
Optimized DNA sequence encoding Human ITAC (CXCL11) mature chain was expressed in Escherichia Coli.
Molecular weight
Human ITAC, generated by the proteolytic removal of the signal peptide and propeptide and has a calculated molecular mass of approximately8 kDa. RecombinantCXCL11 is a monomeric protein consisting of amino acid residue subunits and migrates as an approximately8 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Purity
>98%, as determined by SDS-PAGE and HPLC
Biological Activity
Determined by its ability to chemoattract human T cells using a concentration range of.0-10.0 ng/ml.
Protein Sequence
MSVKGMAIAL AVILCATVVQ GFPMFKRGRC LCIGPGVKAV KVADIEKASI MYPSNNCDKI EVIITLKENK GQRCLNPKSK QARLIIKKVE RKNF
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant ITAC was lyophilized from a 0.2 μm filteredmM PB,100mM NaCl solution pH.5.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
P51677
Interactor
P49682
Biological Process
Biological Process
Molecular function
Methods
ERK1/2 activation in NB cell lines.
- Immunobloting of phospho-ERK (pERK) and total ERK in transduced cells, treated with 100 ng/ml human recombinant CXCL12, in presence or in absence of 1 µM of the CXCR4 blocker TN14003, 100 ng/ml human recombinant CXCL11.
