Human IP-10 Recombinant

Human IP-10 Recombinant

$70.00$2,700.00


accession P02778


Source Optimized DNA sequence encoding Human IP-10 mature chain was expressed in Escherichia Coli.
Molecular weight Native human IP-10/CXCL10, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 9 kDa. RecombinantIP-10 is a monomer protein consisting of 77 amino acid residue subunits, and migrates as an approximately 9 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity >97%, as determined by SDS-PAGE and HPLC
Biological Activity Determined by its ability to chemoattract human T-Lymphocytes using a concentration of-50 ng/ml.

Protein Sequence MNQTAILICC LIFLTLSGIQ GVPLSRTVRC TCISISNQPV NPRSLEKLEI IPASQFCPRV EIIATMKKKG EKRCLNPESK AIKNLLKAVS KERSKRSP
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation RecombinantIP-10/CXCL10 was lyophilized from.2 μm filtered PBS solution, pH7.0.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor P51677
Interactor P49682
Biological Process Chemotaxis
Biological Process Inflammatory-response
Molecular function Cytokine

Methods

Chemokine transcripts induced in human islet cells in response to inflammatory stimuli (microarray analysis).

recombinant human CXCL10
  • Cytokine cocktail -induced expression by a factor of >30 was observed for CCL5, CCL8, CCL22, CX3CL1, CXCL9, and CXCL10 (asterisks indicate significant differences between control and cytokine-treated islets).

PF4, collagen type I and IP-10 present in PEA material.

600 ng/ml IP-10
  • Immunohistochemical stainings for PF4 , trichrome staining and IP-10 .

PF4, collagen type I and IP-10 present in PEA material.

600 ng/ml IP-10
  • Immunohistochemical stainings for PF4 , trichrome staining and IP-10 .

Inhibition of USP18 exacerbates IFNα-induced chemokine expression in INS-1E cells, primary rat beta cells and human-dispersed islet cells.

Human CXCL10
  • CXCL10, CCL5 and IL-15 were assayed by RT-PCR and normalized for the housekeeping genes GAPDH (a, b) or β-actin (c).

Generation and characterization of recombinant IP10-scFv.

the refolded IP10-scFv
  • aThe IP10-scFv gene was inserted into GV219 under the control of the PCMV promoter as a XholI/EcoRI fragment and amplified from the plasmid GV219IP10-scFv.

Cellular migration assays in vitro

  • Migration assays using the IP10-scFv fusion protein were performed in a 24-well Transwell chamber (5-μm pore size ) coated with 10 mg/ml of fibronectin on the bottom of the upper chamber.
  • The DC-induced CTLs were suspended in RPMI 1640 containing 1 % BSA and applied at a density of 1 × 105/well.
  • Then, 200 μl of the cell suspension was placed into the upper Transwell chamber and 100 μl (1 ng/μl) aliquots of the refolded IP10-scFv, 100 ng recombinant human IP-10 , 100 ng anti-EGFRvIII monoclonal antibody , and PBS serially diluted in chemotaxis medium (RPMI1640 with 1 % BSA) were placed in the lower chamber.
  • The chambers were then incubated for 3 h at 37 °C in a humid atmosphere of 5 % CO2.
  • After incubation, the number of cells that migrated to the lower chamber and mixed uniformly was determined with crystal violet staining followed by counting under a light microscope (16 selected high-power fields).
  • The results are expressed as the…
  • Migration assays using the IP10-scFv fusion protein were performed in a 24-well Transwell chamber (5-μm pore size ) coated with 10 mg/ml of fibronectin on the bottom of the upper chamber.
  • The DC-induced CTLs were suspended in RPMI 1640 containing 1 % BSA and applied at a density of 1 × 105/well.
  • Then, 200 μl of the cell suspension was placed into the upper Transwell chamber and 100 μl (1 ng/μl) aliquots of the refolded IP10-scFv, 100 ng recombinant human IP-10 , 100 ng anti-EGFRvIII monoclonal antibody , and PBS serially diluted in chemotaxis medium (RPMI1640 with 1 % BSA) were placed in the lower chamber.
  • The chambers were then incubated for 3 h at 37 °C in a humid atmosphere of 5 % CO2.
  • After incubation, the number of cells that migrated to the lower chamber and mixed uniformly was determined with crystal violet staining followed by counting under a light microscope (16 selected high-power fields).
  • The results are expressed as the chemotaxis index, which was calculated using the following formula: chemotaxis index = migration in response to chemokine / migration to control medium.
  • The index represents the mean of the migration performed in triplicate.

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