Human Interleukin-9 Recombinant

Human Interleukin-9 Recombinant

$70.00$4,700.00


accession P15248


Source Optimized DNA sequence encoding human Interleukin-9 mature chain was expressed in Escherichia Coli.
Molecular weight Native human Interleukin-9 is generated by the proteolytic removal of the signal peptide and propeptide. This molecule has a calculated molecular mass of approximately 14kDa. Recombinant human Interleukin-9 is a monomer protein consisting of 127 amino acid residue subunits, and migrates as an approximately 14 kDa protein under reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity The ED(50) was determined by the dose-dependent proliferation of human MO7e cells is <.4 ng/ml.

Protein Sequence MLLAMVLTSA LLLCSVAGQG CPTLAGILDI NFLINKMQED PASKCHCSAN VTSCLCLGIP SDNCTRPCFS ERLSQMTNTT MQTRYPLIFS RVKKSVEVLK NNKCPYFSCE QPCNQTTAGN ALTFLKSLLE IFQKEKMRGM RGKI
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant human Interleukin-9 was lyophilized from a.2 μm filtered PBS solution.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor Q01113
Molecular function Cytokine
Molecular function Growth-factor

Methods

Plasma levels of IL-9, -17, and -4 in Dengue patients and control groups.

recombinant human IL-9
  • Plasma levels of IL-9 , -17 , and -4 were examined for Dengue patients (DF, DHF and DSS) and control groups (febrile illness and healthy subjects) by ELISA.