Human Interleukin-8 (77AA) Recombinant

/, CXC chemokines/Human Interleukin-8 (77AA) Recombinant

accession P10145


Source Optimized DNA sequence encoding Human Interleukin-8 mature chain was expressed in Escherichia Coli.
Molecular weight Native human Interleukin-8, generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated molecular mass of approximately kDa. Recombinant Interleukin-8 is a monomer protein consisting of78 amino acid residue subunits, migrates as an approximately9 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity Activity was determined by the ability to chemoattract humanpheripheral blood monocyteswas found to be in the range of.0-100.0 ng/ml.

Protein Sequence MTSKLAVALL AAFLISAALC EGAVLPRSAK ELRCQCIKTY SKPFHPKFIK ELRVIESGPH CANTEIIVKL SDGRELCLDP KENWVQRVVE KFLKRAENS
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Interleukin-8 was lyophilized from a.2 μm filtered PBS solution pH7.0.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor P25024
Interactor P25025
Interactor Q16570
Biological Process Chemotaxis
Biological Process Inflammatory-response
Molecular function Cytokine

Methods