Human Interleukin-7 Recombinant (CHO cell)
Categories: CytokinesRecombinant Human Cytokines$160.00 – $4,700.00
Description
Accession
P13232
Source
Optimized DNA sequence encoding Human Interleukin-7 mature chain was expressed in CHO Cells.
Molecular weight
Native human Interleukin-7 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately17 kDa. Recombinant IL-7 is a monomeric protein consisting of153 amino acid residue subunits, and migrates as an approximately17 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Purity
>97%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) determinedby dose-dependent stimulation of the proliferation of murineE8 cells is ≤.5 ng/ml, corresponding to a specific activity of ≥ x units/mg.
Protein Sequence
MFHVSFRYIF GLPPLILVLL PVASSDCDIE GKDGKQYESV LMVSIDQLLD SMKEIGSNCL NNEFNFFKRH ICDANKEGMF LFRAARKLRQ FLKMNSTGDF DLHLLKVSEG TTILLNCTGQ VKGRKPAALG EAQPTKSLEE NKSLKEQKKL NDLCFLKRLL QEIKTCWNKI LMGTKEH
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Recombinant Interleukin-7 was lyophilized from a.2 μm filtered PBS solution pH7.5.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Molecular function
Molecular function