Human Interleukin-6 Recombinant (E.coli)

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Human Interleukin-6 Recombinant (E.coli)

$70.00$2,700.00


accession P05231


Source Optimized DNA sequence encoding Human Interleukin-6 mature chain was expressed in E.Coli
Molecular weight Native human Interleukin-6 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately kDa. Recombinant IL-6 is a homodimer protein consisting of amino acid residue subunits, and migrates as an approximately kDa protein under reducing conditions in SDS-PAGE.
Purity >97%, as determined by SDS-PAGE and HPLC
Biological Activity The ED(50) was determined by the dose-dependent stimulation of the proliferation ofmurineTD1 cells was found to be less than.1 ng/ml, corresponding to specific activity ofx107 IU/mg.
Protein Sequence MNSFSTSAFG PVAFSLGLLL VLPAAFPAPV PPGEDSKDVA APHRQPLTSS ERIDKQIRYI LDGISALRKE TCNKSNMCES SKEALAENNL NLPKMAEKDG CFQSGFNEET CLVKIITGLL EFEVYLEYLQ NRFESSEEQA RAVQMSTKVL IQFLQKKAKN LDAITTPDPT TNASLLTKLQ AQNQWLQDMT THLILRSFKE FLQSSLRALR QM
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Interleukin-6 was lyophilized from a.2 μm filtered PBS solution pH.0.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor P01730 CD4_HUMAN
Interactor P08887 IL6RA_HUMAN
Interactor P08887 IL6RA_HUMAN
Interactor P13725 ONCM_HUMAN
Interactor P40189 IL6RB_HUMAN
Biological Process Acute-phase
Molecular function Cytokine
Molecular function Growth-factor

Methods

Ezrin KD blocks Stat3 phosphorylation, VEGF-A/-C, and IL-6 expression.

Recombinant human IL-6
  • Immunoblot analysis of IL-6 in cell lysates from MDA231 and MDASrc cells expressing the empty vector pLKO.1 or shEZR vector.

Characterization of the detoxified vaccine antigen hIL-1b(D145K).

Recombinant human IL-6
  • (d) IL-6 secretion.

The neoplastic area of CRC samples is massively infiltrated with Th17-related cytokine-, TNF-α- and IL-6-producing cells.

IL-6
  • (a) IFN-γ, IL-17A, IL-17F, IL-21, IL-22, TNF-α and IL-6 proteins were analyzed by enzyme-linked immunosorbent assay in LPMC-derived supernatants (LPMC SNs) and TIL-derived supernatants (TL SNs), and data are expressed as pg/ml supernatants.

Isolation and transduction of primary murine stem- and progenitor cells

  • Hematopoietic stem- and progenitor cells (negative for the mature lineage markers Mac1, Gr1, CD3, CD4, CD8, B220, Ter119, and positive for the primitive surface markers c-Kit and Sca1; LKS +) were FACS-sorted from the bone marrow of C57Bl6 wild-type mice and pre-stimulated with 50 ng/ml murine Stem Cell Factor (mSCF, , ), 10 ng/ml murine Interleukin 3 (mIL-3), and 50 ng/ml human IL-6 at 37 °C, 5% CO2 for 48 h to induce cycling.
  • Pre-stimulated cells were subsequently cultured in the above described cytokines with the addition of 8 µg/ml Polybrene on retronectin-coated plates pre-loaded with viral supernatant (1–2 hits), and GFP+ cells were isolated after an additional 48 h (n = 6 independent transductions).

Selection of affibody molecules from a naïve synthetic library

  • Human IL-6 (hIL-6), was biotinylated using No-Weigh Sulfo-NHS-LC-Biotin and dialyzed to remove unbound biotin.
  • A combinatorial library of affibody molecules displayed on bacteriophage was subjected to four rounds of selection using biotinylated hIL-6 (bio-hIL-6) essentially according to Grönwall et al.Table S1).
  • The DNA sequences of the enriched clones were determined via PCR amplification of insert sequences and using an ABI PRISM® 3130xl Genetic Analyzer instrument (PE Applied Biosystems) according to the manufacturer's instructions.