Human Interleukin-6 Recombinant (CHO Cells)
$160.00 – $3,500.00
| accession | P05231 |
| Source | Optimized DNA sequence encoding Human Interleukin-6 mature chain was expressed in CHO Cells |
| Molecular weight | Native human Interleukin-6 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 21kDa. Recombinant IL-6 is a glycosylated protein consisting of 184 amino acid residue subunits, due to glycosylation migrates as an approximately 30kDa protein under reducing conditions in SDS-PAGE. |
| Purity | >97%, as determined by SDS-PAGE and HPLC |
| Biological Activity | The ED(50) was determined by the dose-dependent stimulation of the proliferation ofmurineTD1cells was found to be less than.1 ng/ml, corresponding to specific activity of 1x107 IU/mg. |
| Protein Sequence | MNSFSTSAFG PVAFSLGLLL VLPAAFPAPV PPGEDSKDVA APHRQPLTSS ERIDKQIRYI LDGISALRKE TCNKSNMCES SKEALAENNL NLPKMAEKDG CFQSGFNEET CLVKIITGLL EFEVYLEYLQ NRFESSEEQA RAVQMSTKVL IQFLQKKAKN LDAITTPDPT TNASLLTKLQ AQNQWLQDMT THLILRSFKE FLQSSLRALR QM |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
| Presentation | Interleukin-6 was lyophilized from a 0.2 μm filtered PBS solution pH7.0. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers |
| Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | P01730 CD4_HUMAN |
| Interactor | P08887 IL6RA_HUMAN |
| Interactor | P08887 IL6RA_HUMAN |
| Interactor | P13725 ONCM_HUMAN |
| Interactor | P40189 IL6RB_HUMAN |
| Biological Process | Acute-phase |
| Molecular function | Cytokine |
| Molecular function | Growth-factor |