Human Interleukin-5 Recombinant

Human Interleukin-5 Recombinant

$70.00$4,700.00


accession P05113


Source Optimized DNA sequence encoding Human Interleukin-5 mature chain was expressed in Escherichia Coli.
Molecular weight Nativehuman Interleukin-5, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately13 kDa. Recombinant IL-5 is a disulfide-linked homodimeric protein consisting of two amino acid residue subunits,and migrates as an approximately26 kDa protein under non-reducing conditions and as a13 kDa protein under reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity The ED(50) was determined by the dose-dependent proliferation of TF-1 cells was ≤.2 ng/ml, corresponding to a specific activity of ≥ x units/mg.
Protein Sequence MRMLLHLSLL ALGAAYVYAI PTEIPTSALV KETLALLSTH RTLLIANETL RIPVPVHKNH QLCTEEIFQG IGTLESQTVQ GGTVERLFKN LSLIKKYIDG QKKKCGEERR RVNQFLDYLQ EFLGVMNTEW IIES
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant Interleukin-5 was lyophilized from a.2 μm filtered PBS solution pH.5.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor P32927 IL3RB_HUMAN
Interactor Q01344 IL5RA_HUMAN
Molecular function Cytokine
Molecular function Growth-factor

Methods

Functional SMEZ is not required for production of inflammatory cytokines in superantigen-sensitive mice.

IL-5
  • In contrast, GAS-M3

Isolation of PB and BM Eos

  • PB Eos were isolated using anti-CD16-conjugated magnetic beads following the manufacturer's protocol.
  • Briefly, blood granulocytes were recovered from whole blood via Percoll density centrifugation.
  • Subsequent incubation of these granulocytes with anti-CD16-conjugated magnetic beads for negative selection led to the retention of CD16+ neutrophils in a MACS column and recovery of untouched, CD16 PB eosinophils.
  • BM Eos were purified from aspirates as described previously.in vitro 8-day culture in a pro-Eos-survival medium (RPMIeos) containing 1 ng/ml recombinant IL-5 .