Human Interleukin-4 Recombinant (CHO cell)

////Human Interleukin-4 Recombinant (CHO cell)

Human Interleukin-4 Recombinant (CHO cell)

$160.00$4,700.00


accession P05112


Source Optimized DNA sequence encoding Human Interleukin-4 mature chain was expressed in CHO Cells.
Molecular weight Native human Interleukin-4, generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated molecular mass of approximately 15 kDa. Recombinant Interleukin-4 is a protein consisting of 130 amino acid residue subunits, due to glycosylation migrates as an approximately 19 kDa protein under non-reducingand reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity The ED(50) was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤.1 ng/ml, corresponding to a specific activity of >x units/mg.
Protein Sequence MGLTSQLLPP LFFLLACAGN FVHGHKCDIT LQEIIKTLNS LTEQKTLCTE LTVTDIFAAS KNTTEKETFC RAATVLRQFY SHHEKDTRCL GATAQQFHRH KQLIRFLKRL DRNLWGLAGL NSCPVKEANQ STLENFLERL KTIMREKYSK CSS
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Interleukin-4 was lyophilized from a.2 μm filtered solution in.5% glycine,.5% sucrose,.01% Tween80, mM Glutamic acid, pH.5.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor P24394 IL4RA_HUMAN
Biological Process B-cell-activation
Molecular function Cytokine
Molecular function Growth-factor