Human Interleukin-4 Recombinant, Biotinylated

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Human Interleukin-4 Recombinant, Biotinylated

$160.00$4,700.00


accession P05112


Source DNA sequence encoding Human IL4 including a C-terminal AVI polyHis tag was expressed in HEK293 cells. Recombinant Human IL4 was further labeled by Biotin for detection by avidin conjugated secondary probes.
Molecular weight Recombinant Human IL4 is a protein consisting of amino acid residues [Met23-Ser153] with a theoretical MW of 15kD. Recombinant IL-4 migrates as an approximately 20 kDa protein on SDS-PAGE due to glycosylation.
Bioactivity The activity was tested by the ability to bind human IL4R in an ELISA assay at a linear range of 2-10 ng/ml.
Purity >95%, as determined by SDS-PAGE and HPLC
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant IL4 was lyophilized from 0.2 µm filtered PBS solution pH.4
Sequence MGLTSQLLPP LFFLLACAGN FVHGHKCDIT LQEIIKTLNS LTEQKTLCTE LTVTDIFAAS KNTTEKETFC RAATVLRQFY SHHEKDTRCL GATAQQFHRH KQLIRFLKRL DRNLWGLAGL NSCPVKEANQ STLENFLERL KTIMREKYSK
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor P24394 IL4RA_HUMAN
Biological Process B-cell-activation
Molecular function Cytokine
Molecular function Growth-factor