Human Interleukin-4 Recombinant, Biotinylated
$160.00 – $4,700.00
| accession | P05112 |
| Source | DNA sequence encoding Human IL4 including a C-terminal AVI polyHis tag was expressed in HEK293 cells. Recombinant Human IL4 was further labeled by Biotin for detection by avidin conjugated secondary probes. |
| Molecular weight | Recombinant Human IL4 is a protein consisting of amino acid residues [Met23-Ser153] with a theoretical MW of 15kD. Recombinant IL-4 migrates as an approximately 20 kDa protein on SDS-PAGE due to glycosylation. |
| Bioactivity | The activity was tested by the ability to bind human IL4R in an ELISA assay at a linear range of 2-10 ng/ml. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
| Presentation | Recombinant IL4 was lyophilized from 0.2 µm filtered PBS solution pH7.4 |
| Sequence | MGLTSQLLPP LFFLLACAGN FVHGHKCDIT LQEIIKTLNS LTEQKTLCTE LTVTDIFAAS KNTTEKETFC RAATVLRQFY SHHEKDTRCL GATAQQFHRH KQLIRFLKRL DRNLWGLAGL NSCPVKEANQ STLENFLERL KTIMREKYSK |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. |
| Usage | This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | P24394 IL4RA_HUMAN |
| Biological Process | B-cell-activation |
| Molecular function | Cytokine |
| Molecular function | Growth-factor |