Human Interleukin-33 Recombinant
$70.00 – $4,700.00
accession | O95760 |
Source | Optimized DNA sequence encoding Human Interleukin-33 mature chain was expressed in Escherichia Coli. |
Molecular weight | Nativehuman Interleukin-33, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 18kDa. Recombinant Interleukin-33 is a monomer protein consisting of 159 amino acid residue subunits,and migrates as an approximately 18kDa protein under non-reducing and reducing conditions in SDS-PAGE. |
Purity | >95%, as determined by SDS-PAGE and HPLC |
Biological Activity | The ED(50) was determined by the dose-dependent proliferation ofmurine D10S cells is ≤.03 ng/ml. |
Protein Sequence | MKPKMKYSTN KISTAKWKNT ASKALCFKLG KSQQKAKEVC PMYFMKLRSG LMIKKEACYF RRETTKRPSL KTGRKHKRHL VLAACQQQST VECFAFGISG VQKYTRALHD SSITGISPIT EYLASLSTYN DQSITFALED ESYEIYVEDL KKDEKKDKVL LSYYESQHPS NESGDGVDGK MLMVTLSPTK DFWLHANNKE HSVELHKCEK PLPDQAFFVL HNMHSNCVSF ECKTDPGVFI GVKDNHLALI KVDSSENLCT ENILFKLSET |
Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
Presentation | Recombinant Interleukin-33 was lyophilized from0.2 μm filtered1mg/ml in PBS buffer. |
Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
Interactor | Q01638 ILRL1_HUMAN |
Molecular function | Cytokine |
Methods
Cell sorting yields a purified ILCs that produces Th2-, Th17- and Th1-related cytokines. |