Human Interleukin-31 Recombinant
$70.00 – $4,700.00
| accession | Q6EBC2 |
| Source | Optimized DNA sequence encoding Human Interleukin-31 mature chain was expressed in Escherichia Coli. |
| Molecular weight | Native human Interleukin-31, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 16kDa. Recombinant Interleukin-31 is a monomer protein consisting of 141 amino acid residue subunits,and migrates as an approximately 16kDa protein under non-reducing and reducing conditions in SDS-PAGE. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Biological Activity | The ED(50) was determined by the ability to induce STAT3 activation in U87 MG cells, and was determined to be not less thanng/ml. |
| Protein Sequence | MASHSGPSTS VLFLFCCLGG WLASHTLPVR LLRPSDDVQK IVEELQSLSK MLLKDVEEEK GVLVSQNYTL PCLSPDAQPP NNIHSPAIRA YLKTIRQLDN KSVIDEIIEH LDKLIFQDAP ETNISVPTDT HECKRFILTI SQQFSECMDL ALKSLTSGAQ QATT |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
| Presentation | Recombinant Interleukin-33 was lyophilized from a 0.2 μm filtered PBS solution pH7.5. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Biological Process | Immunity |
| Molecular function | Cytokine |