Human Interleukin-31 Recombinant

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Human Interleukin-31 Recombinant

$70.00$4,700.00


accession Q6EBC2


Source Optimized DNA sequence encoding Human Interleukin-31 mature chain was expressed in Escherichia Coli.
Molecular weight Native human Interleukin-31, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 16kDa. Recombinant Interleukin-31 is a monomer protein consisting of 141 amino acid residue subunits,and migrates as an approximately 16kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity The ED(50) was determined by the ability to induce STAT3 activation in U87 MG cells, and was determined to be not less thanng/ml.

Protein Sequence MASHSGPSTS VLFLFCCLGG WLASHTLPVR LLRPSDDVQK IVEELQSLSK MLLKDVEEEK GVLVSQNYTL PCLSPDAQPP NNIHSPAIRA YLKTIRQLDN KSVIDEIIEH LDKLIFQDAP ETNISVPTDT HECKRFILTI SQQFSECMDL ALKSLTSGAQ QATT
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant Interleukin-33 was lyophilized from a.2 μm filtered PBS solution pH7.5.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Biological Process Immunity
Molecular function Cytokine