Human Interleukin-3 Recombinant (CHO cell)

////Human Interleukin-3 Recombinant (CHO cell)

Human Interleukin-3 Recombinant (CHO cell)

$160.00$3,500.00

SKU: RCP08700 Category: Tags: , , , ,

accession P08700


Source Optimized DNA sequence encoding Human Interleukin-3 mature chain was expressed in CHO cells.
Molecular weight Native human Interleukin-3 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 15kDa. Recombinant IL-3 is a protein consisting of 133 amino acid residue subunits, and due to glycosylation migrates as an approximately 20kDa protein under reducing conditions in SDS-PAGE.
Purity >97%, as determined by SDS-PAGE and HPLC
Biological Activity The ED(50) was determined by the the ability to induce IFNg production in NK cells was foundless than0.1 ng/ml.

Protein Sequence MSRLPVLLLL QLLVRPGLQA PMTQTTPLKT SWVNCSNMID EIITHLKQPP LPLLDFNNLN GEDQDILMEN NLRRPNLEAF NRAVKSLQNA SAIESILKNL LPCLPLATAA PTRHPIHIKD GDWNEFRRKL TFYLKTLENA QAQQTTLSLA IF
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant Interleukin-3 was lyophilized from a.2 μm filtered PBS solution pH7.0.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor P26951 IL3RA_HUMAN
Interactor P32927 IL3RB_HUMAN
Molecular function Cytokine
Molecular function Growth-factor