Human Interleukin-22 Recombinant (E.Coli)
$70.00 – $4,700.00
| accession | Q9GZX6 |
| Source | Optimized DNA sequence encoding Human Interleukin-22 mature chain was expressed in Escherichia Coli. |
| Molecular weight | Native human IL-22 has a calculated molecular mass of approximately 17 kDa. Recombinant IL-22 is a disulfide-linked homodimer protein consisting of 2x147 amino acid residue subunits, andm igrates as an approximately 17 kDa protein under reducing conditions in SDS-PAGE. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Biological Activity | Activitywas determined by the ability to inhibit growth of human A549 cancer cells at concentrations ranging between-100 ng/ml. |
| Protein Sequence | MAALQKSVSS FLMGTLATSC LLLLALLVQG GAAAPISSHC RLDKSNFQQP YITNRTFMLA KEASLADNNT DVRLIGEKLF HGVSMSERCY LMKQVLNFTL EEVLFPQSDR FQPYMQEVVP FLARLSNRLS TCHIEGDDLH IQRNVQKLKD TVKKLGESGE IKAIGELDLL FMSLRNACI |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
| Presentation | Recombinant Interleukin-22 was lyophilized from.2μm filtered concentrated (1.0mg/ml) solution inmM NaCl,mM Tris, pH7.0. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | Q8N6P7 I22R1_HUMAN |
| Interactor | Q08334 I10R2_HUMAN |
| Interactor | Q969J5-2 |
| Interactor | Q969J5-3 |
| Molecular function | Cytokine |
Methods
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IL-22 enhances miR-197 expression.
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The neoplastic area of CRC samples is massively infiltrated with Th17-related cytokine-, TNF-α- and IL-6-producing cells.
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