Human Interleukin-1 alpha Recombinant (CHO Cell)
Categories: CytokinesRecombinant Human Cytokines$160.00 – $4,700.00
Description
Accession
P01583
Source
Optimized DNA sequence encoding Human Interleukin-1 alpha mature chain was expressed in CHO Cells.
Molecular weight
Mature human IL-1 alpha, is generated by the proteolytic removal of the signal peptide and propeptide. The molecule has a calculated molecular mass of approximately 18 kDa. Recombinant Interleukin-1a is a monomer protein consisting of 160 amino acid residue subunits, due to glycosylation migrates as an approximately 25 kDa protein under reducing conditions in SDS-PAGE.
Purity
>97%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by thedose-dependent stimulation of murine D10S cells is ≤.002 ng/ml, corresponding to a specific activity of ≥2 x units/mg.
Protein Sequence
MAKVPDMFED LKNCYSENEE DSSSIDHLSL NQKSFYHVSY GPLHEGCMDQ SVSLSISETS KTSKLTFKES MVVVATNGKV LKKRRLSLSQ SITDDDLEAI ANDSEEEIIK PRSAPFSFLS NVKYNFMRII KYEFILNDAL NQSIIRANDQ YLTAAALHNL DEAVKFDMGA YKSSKDDAKI TVILRISKTQ LYVTAQDEDQ PVLLKEMPEI PKTITGSETN LLFFWETHGT KNYFTSVAHP NLFIATKQDY WVCLAGGPPS ITDFQILENQ A
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Interleukin-1 alphawas lyophilized from a.2 μm filtered PBS solution pH7.0.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function
Molecular function
Molecular function
