Human Interleukin-2 His Recombinant
Categories: HematopoietinsIL-2 familyRecombinant Human Cytokines$160.00 – $1,100.00
Description
Accession
P60568
Source
Optimized DNA sequence encoding Human Interleukin-2 mature chain fused at C terminal to His tag was expressed in HEK293.
Molecular weight
Recombinant Interleukin-2 his, is a protein consisting of 140 amino acid residue subunits, and migrates as an approximately 30kDa protein due to glycosylation under reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by the dose-dependent proliferation of mouse CTLL2 cells was found to be in the range of.0-80.0 ng/ml.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human Interleukin-2 was lyophilized from a 0.2 μm filtered PBS solution.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Biological Process
Molecular function
Molecular function
