Human Interleukin-15 Recombinant, Biotinylated

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Human Interleukin-15 Recombinant, Biotinylated


accession P40933

Source DNA sequence encoding Human Interleukin-15 mature chain fused to an AVI IgG1 C-terminal tag was expressed in HEK292 cells.
Molecular weight Native human Interleukin-15 is generated by the proteolytic removal of the signal peptide and propeptide with a theoretical molecular mass of approximately 13kDa. Recombinant Interleukin-15 Fc chimera is a homodimer protein and migrates an approximately as a 41KDa protein under reducing conditions in SDS-PAGE.
Purity >96%, as determined by SDS-PAGE and HPLC
Biological Activity Activity was determined by the binding of human IL-15 receptor alpha chain in a functional ELISA assay in a linear range of 1-100 ng/ml.
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant IL-15 was lyophilized from a.2 μm filtered PBS solution pH7.5 .
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Interactor P14784 IL2RB_HUMAN
Interactor P31785 IL2RG_HUMAN
Interactor P41273 TNFL9_HUMAN
Molecular function Cytokine