Human Interleukin-10 Recombinant (CHO cell)
$160.00 – $4,700.00
| accession | P22301 |
| Source | Optimized DNA sequence encoding Human Interleukin-10 mature chain was expressed in CHO Cell |
| Molecular weight | Native Human Interleukin-10 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 19 kDa. Recombinant Human Interleukin-10 is a protein consisting of 161 amino acid residue subunits, and due to glycosylation migrates as an approximately 25 kDa protein under reducing conditions in SDS-PAGE. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Biological Activity | The ED50 determined by dose-dependent co-stimulation (with murine IL-4) of MC/9 cells. The ED50 was found to be ≤ ng/ml, corresponding to a specific activity of ≥x units/mg. |
| Protein Sequence | MHSSALLCCL VLLTGVRASP GQGTQSENSC THFPGNLPNM LRDLRDAFSR VKTFFQMKDQ LDNLLLKESL LEDFKGYLGC QALSEMIQFY LEEVMPQAEN QDPDIKAHVN SLGENLKTLR LRLRRCHRFL PCENKSKAVE QVKNAFNKLQ EKGIYKAMSE FDIFINYIEA YMTMKIRN |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
| Presentation | Recombinant Interleukin-10 was lyophilized from a.2 μm filtered PBS solution pH7.0. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | Q13651 I10R1_HUMAN |
| Interactor | Q08334 I10R2_HUMAN |
| Molecular function | Cytokine |