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Human Interleukin 1 beta Recombinant

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$70.00$3,500.00

SKU: RKP01584 Tags: , , , , ,

Description

Accession
P01584
Source
Optimized DNA sequence encoding Human Interleukin-1 beta mature chain was expressed in Escherichia Coli.
Molecular weight
Mature Human IL-1 beta, is generated by the proteolytic removal of the signal peptide and propeptide.The molecule has a calculated molecular mass of approximately17 kDa. Recombinant IL-1 beta is a monomer protein consisting of 154 amino acid residue subunits. Recombinant IL-1 beta migrates as an approximately 17 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity
>97%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) as determined by thedose-dependent proliferation of murine D10S cells, was found to be ≤0.1 pg/ml, corresponding to a specific activity of ≥1 x107units/mg.
Protein Sequence
MAEVPELASE MMAYYSGNED DLFFEADGPK QMKCSFQDLD LCPLDGGIQL RISDHHYSKG FRQAASVVVA MDKLRKMLVP CPQTFQENDL STFFPFIFEE EPIFFDTWDN EAYVHDAPVR SLNCTLRDSQ QKSLVMSGPY ELKALHLQGQ DMEQQVVFSM SFVQGEESND KIPVALGLKE KNLYLSCVLK DDKPTLQLES VDPKNYPKKK MEKRFVFNKI EINNKLEFES AQFPNWYIST SQAENMPVFL GGTKGGQDIT DFTMQFVSS
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
IL-1 beta was lyophilized from a.2 μm filtered PBS pH7.0.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
P29466
Biological Process
Molecular function
Molecular function
Molecular function

Methods

Materials

  • IFN-α and -β were from PBL Medical Laboratories (Piscataway, ), and IFN-λ1 (interleukin-29) was from .
  • TNF-α was from Prospec-Tany .
  • Interleukin-1β (IL-1β) and IFN-γ were from & .
  • All of the cytokines were produced by recombinant expression of human genes.
  • The functional activities of the IFNs were confirmed by demonstrating their abilities to induce IFN signaling, as indicated by translocation of STAT2 from the cytoplasm to the nuclei of treated cells (

Materials

  • IFN-α and -β were from PBL Medical Laboratories (Piscataway, ), and IFN-λ1 (interleukin-29) was from .
  • TNF-α was from Prospec-Tany .
  • Interleukin-1β (IL-1β) and IFN-γ were from & .
  • All of the cytokines were produced by recombinant expression of human genes.
  • The functional activities of the IFNs were confirmed by demonstrating their abilities to induce IFN signaling, as indicated by translocation of STAT2 from the cytoplasm to the nuclei of treated cells (

Effect of anti-miR-146a LNA or miR-146a mimic upon IL-1β-induced release of inflammatory molecules.

  • In comparison with IL-1ß alone, cultures stimulated with IL-1ß and transfected with LNA-anti miR-146a exhibited significant increase of IL-6 and IP-10 release, whereas transfection of glial cells with miR-146a mimic significantly decreased the levels of IL-6, IL-8, G-CSF, IFN-γ, IP-10, MIP-1β, and TNF-α (* p<0.05).

CCR2 expression on blood DCs is reduced in T1D.

  • Overnight incubation with LPS, IL-1β, or CL097 induced a clear decrease in CCR2 expression, as measured by flow cytometry, demonstrating that CCR2 expression on human mDCs is downregulated by various DC-maturing stimuli.

Identification of IL-1α and IL-1β as Ep-derived FSA and alarmin.

  • Conditioned medium of control Ep cultures (Ep-PDT) and PDT-treaded Ep cultures (Ep+PDT), whole-cell homogenate of control Ep and Sephadex G-150-purified 17 kD FSA were reacted with antibodies against IL-1α or IL-1β as indicated and then tested for the ability to activate signalling , to enhance IL-6 that induces GFP expression in H-35 cells and to increase neutrophil binding .

Regents and materials

  • Recombinant human interleukin-1β (IL-1β) was from .
  • Human IL-1ra and HSA were cloned and produced in Pichia pastoris in our laboratory using the same expression vectors and host cells as described in this study, with 6 histidines (His tag) in the carboxyl terminal.
  • FITC-labeled anti-His tag monoclonal antibody (mAb) and HRP-conjugated anti-His tag mAb were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).Restriction endonuclease, T4 DNA ligase, DNase, and RNase were from TaKaRa Biotechnology (Dalian, China).
  • Pfu DNA polymerase was a product of .
  • All forward and reverse primers were purchase from .
  • Ni-NTA agarose was from .
  • Bovine type II collagen and Complete Freund’s adjuvant were obtained from .
  • The commercial ELISA kit for mouse TNF-α, IL-1β, IL-6 and albumin were from .

Dose-dependent inhibition of the cytotoxic effect of IL-1β to A375.S2 cells.

  • Cells were incubated in the presence of 32 nM fusion protein (1), 1 ng/ml (0.06nM) IL-1β alone (2), 0.06nM IL-1β plus 2 nM fusion protein (3), and 0.06nM IL-1β plus 32 nM fusion protein (4).

Effects of the curcuma DMSO extract (2a) and curcuma ethanol extract (2b) on mRNA levels of candidate genes after 6 hours, indicated as fold change relative to IL-1β-treatment (IL-1β treated cells also contain 0.03%of DMSO or EtOH respectively).

  • Each gene was normalized to its respective IL-1β treatment (IL-1β + solvent), which was always set to 100% (only one exemplary control bar is shown).

Inflammatory conditions reduce Imatinib uptake and hMATE1 expression in hRASF.

  • B, C, and D) Effect of 18 hours incubation with a TNFα, IL-1β and IL-6 (+sIL-6R) cocktail and with single cytokines (each at 10 ng/ml) on Imatinib uptake in hRASF , hMATE1-mRNA expression , hMATE1-protein expression by immunofluorescence staining (upper part of D) and by Western Blot analysis of biotynilated plasma membrane fractions hMATE1 (lower part of D showing an example of a typical Western blot together with the quantitative analysis of three independent experiments).

Kir4.1 and IL-1β expression in rat temporal cortex after status epilepticus (SE).

  • mRNA expression levels of Kir4.1 and IL-1β in the temporal cortex of control rats (n = 6; Con), rats at 1 week (n = 6), and 3 months (n = 6) after SE.
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