Human Interleukin-1 alpha Recombinant (CHO Cell)

////Human Interleukin-1 alpha Recombinant (CHO Cell)

Human Interleukin-1 alpha Recombinant (CHO Cell)

$160.00$4,700.00


accession P01583


Source Optimized DNA sequence encoding Human Interleukin-1 alpha mature chain was expressed in CHO Cells.
Molecular weight Mature human IL-1 alpha, is generated by the proteolytic removal of the signal peptideand propeptide. The molecule has a calculated molecular mass of approximately kDa. Recombinant Interleukin-1a is a monomer protein consisting of160 amino acid residue subunits, due to glycosylation migrates as an approximatelykDa protein under reducing conditions in SDS-PAGE.
Purity >97%, as determined by SDS-PAGE and HPLC
Biological Activity The ED(50) was determined by thedose-dependent stimulation of murine D10S cells is ≤.002 ng/ml, corresponding to a specific activity of ≥2 x units/mg.
Protein Sequence MAKVPDMFED LKNCYSENEE DSSSIDHLSL NQKSFYHVSY GPLHEGCMDQ SVSLSISETS KTSKLTFKES MVVVATNGKV LKKRRLSLSQ SITDDDLEAI ANDSEEEIIK PRSAPFSFLS NVKYNFMRII KYEFILNDAL NQSIIRANDQ YLTAAALHNL DEAVKFDMGA YKSSKDDAKI TVILRISKTQ LYVTAQDEDQ PVLLKEMPEI PKTITGSETN LLFFWETHGT KNYFTSVAHP NLFIATKQDY WVCLAGGPPS ITDFQILENQ A
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Interleukin-1 alphawas lyophilized from a.2 μm filtered PBS solution pH.0.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Biological Process Inflammatory-response
Molecular function Cytokine
Molecular function Mitogen
Molecular function Pyrogen