Human Interleukin-1 alpha Recombinant (CHO Cell)
$160.00 – $4,700.00
| accession | P01583 |
| Source | Optimized DNA sequence encoding Human Interleukin-1 alpha mature chain was expressed in CHO Cells. |
| Molecular weight | Mature human IL-1 alpha, is generated by the proteolytic removal of the signal peptide and propeptide. The molecule has a calculated molecular mass of approximately 18 kDa. Recombinant Interleukin-1a is a monomer protein consisting of 160 amino acid residue subunits, due to glycosylation migrates as an approximately 25 kDa protein under reducing conditions in SDS-PAGE. |
| Purity | >97%, as determined by SDS-PAGE and HPLC |
| Biological Activity | The ED(50) was determined by thedose-dependent stimulation of murine D10S cells is ≤.002 ng/ml, corresponding to a specific activity of ≥2 x units/mg. |
| Protein Sequence | MAKVPDMFED LKNCYSENEE DSSSIDHLSL NQKSFYHVSY GPLHEGCMDQ SVSLSISETS KTSKLTFKES MVVVATNGKV LKKRRLSLSQ SITDDDLEAI ANDSEEEIIK PRSAPFSFLS NVKYNFMRII KYEFILNDAL NQSIIRANDQ YLTAAALHNL DEAVKFDMGA YKSSKDDAKI TVILRISKTQ LYVTAQDEDQ PVLLKEMPEI PKTITGSETN LLFFWETHGT KNYFTSVAHP NLFIATKQDY WVCLAGGPPS ITDFQILENQ A |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
| Presentation | Interleukin-1 alphawas lyophilized from a.2 μm filtered PBS solution pH7.0. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Biological Process | Inflammatory-response |
| Molecular function | Cytokine |
| Molecular function | Mitogen |
| Molecular function | Pyrogen |