Human Interferon-gamma Recombinant (CHO Cell)

///Human Interferon-gamma Recombinant (CHO Cell)

Human Interferon-gamma Recombinant (CHO Cell)

$160.00$1,250.00


accession P01579


Source Optimized DNA sequence encoding Human Interferon gamma mature chain was expressed in CHO Cells
Molecular weight Native Interferon gamma is generated by the proteolytic removal of the signal peptide , the molecule has a calculated molecular mass of approximately 17 kDa. Recombinant Interferon gamma is a monomer protein consisting of 143 amino acid residue subunits, due to glycosylation migrates as an approximately 25 kDa protein under reducing conditions in SDS-PAGE.
Purity >98%, as determined by SDS-PAGE and HPLC
Biological Activity The ED(50) was determined by a cytotoxicity assay of HT29 cells and was found to be ≤ 0.5ng/ml, corresponding to a specific activity of ≥2x107 units/mg.
Protein Sequence MKYTSYILAF QLCIVLGSLG CYCQDPYVKE AENLKKYFNA GHSDVADNGT LFLGILKNWK EESDRKIMQS QIVSFYFKLF KNFKDDQSIQ KSVETIKEDM NVKFFNSNKK KRDDFEKLTN YSVTDLNVQR KAIHELIQVM AELSPAAKTG KRKRSQMLFR GRRASQ
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant Interferon gammawas lyophilized from a.2μm filtered concentrated (1mg/ml) solution in PBS, pH.2.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor P15260 INGR1_HUMAN
Interactor Q66793
Interactor P70380 IL18_MOUSE
Biological Process Antiviral-defense
Biological Process Growth-regulation
Molecular function Cytokine