Human Interferon-gamma Recombinant (CHO Cell)
$160.00 – $1,250.00
| accession | P01579 |
| Source | Optimized DNA sequence encoding Human Interferon gamma mature chain was expressed in CHO Cells |
| Molecular weight | Native Interferon gamma is generated by the proteolytic removal of the signal peptide , the molecule has a calculated molecular mass of approximately 17 kDa. Recombinant Interferon gamma is a monomer protein consisting of 143 amino acid residue subunits, due to glycosylation migrates as an approximately 25 kDa protein under reducing conditions in SDS-PAGE. |
| Purity | >98%, as determined by SDS-PAGE and HPLC |
| Biological Activity | The ED(50) was determined by a cytotoxicity assay of HT29 cells and was found to be ≤ 0.5ng/ml, corresponding to a specific activity of ≥2x107 units/mg. |
| Protein Sequence | MKYTSYILAF QLCIVLGSLG CYCQDPYVKE AENLKKYFNA GHSDVADNGT LFLGILKNWK EESDRKIMQS QIVSFYFKLF KNFKDDQSIQ KSVETIKEDM NVKFFNSNKK KRDDFEKLTN YSVTDLNVQR KAIHELIQVM AELSPAAKTG KRKRSQMLFR GRRASQ |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
| Presentation | Recombinant Interferon gammawas lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in PBS, pH.2. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | P15260 INGR1_HUMAN |
| Interactor | Q66793 |
| Interactor | P70380 IL18_MOUSE |
| Biological Process | Antiviral-defense |
| Biological Process | Growth-regulation |
| Molecular function | Cytokine |