Human Interferon-beta 1a Recombinant
$70.00 – $3,500.00
| accession | P01574 |
| Source | Optimized DNA sequence encoding HumanInterferon beta 1a mature chain was expressed in Chinese Hamster Ovary Cells. |
| Molecular weight | Recombinant human IFN-beta 1a, generated by the proteolytic removal of the signal peptide and propeptide, and has a calculated molecular mass of approximately 19 kDa. Recombinant Interferon beta is a monomeric protein consisting of 166 amino acid residue subunits, and migrates due to glycosylation as an approximately 25kDa protein under reducing conditions in SDS-PAGE. |
| Purity | >98%, as determined by SDS-PAGE and HPLC |
| Biological Activity | Theactivity was determined by theviral resistance assay of Human WISH cells was found to be in the range ofx108 IU/mg. |
| Protein Sequence | MTNKCLLQIA LLLCFSTTAL SMSYNLLGFL QRSSNFQCQK LLWQLNGRLE YCLKDRMNFD IPEEIKQLQQ FQKEDAALTI YEMLQNIFAI FRQDSSSTGW NETIVENLLA NVYHQINHLK TVLEEKLEKE DFTRGKLMSS LHLKRYYGRI LHYLKAKEYS HCAWTIVRVE ILRNFYFINR LTGYLRN |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
| Presentation | Recombinant IFN-bawas lyophilized from a.2 μm filtered sodium acetate solution pH.8. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | P17181 |
| Interactor | P48551 INAR2_HUMAN |
| Biological Process | Antiviral-defense |
| Molecular function | Cytokine |
Methods
Cytokines and Drugs
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