Human Interferon-alpha 2b Recombinant
$70.00 – $880.00
| accession | P01563 |
| Source | Optimized DNA sequence encoding Human Interferon alpha-2b mature chain was expressed in Escherichia Coli. |
| Molecular weight | Native human IFNa-2b is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 19 kDa. Recombinant Interferon-alpha 2b is a monomeric protein consisting of 166 amino acid residue subunits.Interefron alpha 2b migrates as an approximately 19 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. |
| Purity | >98%, as determined by SDS-PAGE and HPLC |
| Biological Activity | The ED(50) was determined by viral resistance assayand was found to benot less than5x108IU/mg. |
| Protein Sequence | MALTFYLLVA LVVLSYKSFS SLGCDLPQTH SLGSRRTLML LAQMRKISLF SCLKDRHDFG FPQEEFGNQF QKAETIPVLH EMIQQIFNLF STKDSSAAWD ETLLDKFYTE LYQQLNDLEA CVIQGVGVTE TPLMKEDSIL AVRKYFQRIT LYLKEKKYSP CAWEVVRAEI MRSFSLSTNL QESLRSKE |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
| Presentation | IFN-alpha2b was lyophilized from a.2 μm filtered PBS solution pH7.0. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | P17181 |
| Interactor | P48551 INAR2_HUMAN |
| Biological Process | Antiviral-defense |
| Molecular function | Cytokine |