Human Interferon-alpha 2b Recombinant

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Human Interferon-alpha 2b Recombinant

$70.00$880.00


accession P01563


Source Optimized DNA sequence encoding HumanInterferonalpha-2bmature chain was expressed in Escherichia Coli.
Molecular weight Native humanIFNa-2bis generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately19 kDa. Recombinant Interferon-alphabis a monomeric protein consisting of amino acid residue subunits.Interefron alphabmigrates as an approximately19 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Purity >98%, as determined by SDS-PAGE and HPLC
Biological Activity The ED(50) was determined by viral resistance assayand was found to benot less than5x108IU/mg.
Protein Sequence MALTFYLLVA LVVLSYKSFS SLGCDLPQTH SLGSRRTLML LAQMRKISLF SCLKDRHDFG FPQEEFGNQF QKAETIPVLH EMIQQIFNLF STKDSSAAWD ETLLDKFYTE LYQQLNDLEA CVIQGVGVTE TPLMKEDSIL AVRKYFQRIT LYLKEKKYSP CAWEVVRAEI MRSFSLSTNL QESLRSKE
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation IFN-alpha2b was lyophilized from a.2 μm filtered PBS solution pH.0.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor P17181
Interactor P48551 INAR2_HUMAN
Biological Process Antiviral-defense
Molecular function Cytokine