Human Insulin Like Growth Factor-2 Recombinant
$70.00 – $1,700.00
| accession | P01344 |
| Source | Optimized DNA sequence encoding Insulin Like Growth Factor-2mature chain was expressed in Escherichia Coli. |
| Molecular weight | Native human Insulin Like Growth Factor-2 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately7 kDa. RecombinantIGF-2 is a monomer protein consisting of68 amino acid residue subunits, and migrates as an approximately7 kDa protein under non-reducing and reducing conditions in SDS-PAGE. |
| Purity | >97%, as determined by SDS-PAGE and HPLC |
| Biological Activity | The ED(50) was determined by the proliferation ofFDC-P1 cells and was found to be is ≤.0 ng/ml, corresponding to a specific activity of ≥1 x units/mg. |
| Protein Sequence | MGIPMGKSML VLLTFLAFAS CCIAAYRPSE TLCGGELVDT LQFVCGDRGF YFSRPASRVS RRSRGIVEEC CFRSCDLALL ETYCATPAKS E RDVSTPPTV LPDNFPRYPV GKFFQYDTWK QSTQRLRRGL PALLRARRGH VLAKELEAFR EAKRHRPLIA LPTQDPAHGG APPEMASNRK |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
| Presentation | Recombinant IGF-2 was lyophilized from a.2 μm filtered PBS solution pH.5. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | P08833 |
| Interactor | P11717 |
| Interactor | P17936 IBP3_HUMAN |
| Biological Process | Carbohydrate-metabolism |
| Biological Process | Osteogenesis |
| Molecular function | Growth-factor |
| Molecular function | Hormone |
| Molecular function | Mitogen |
Methods
IGF2 infusion reduces the number of Aβ40 and Aβ42 plaques in the hippocampus.
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BMP and FGF are robust trophic factors for purified astrocytes.
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