Human IL2RA Recombinant (CD25)
$130.00 – $1,200.00
| accession | P01589 |
| Source | Optimized DNA sequence encoding extracellular domain of human CD25 including a C-terminal polyHis tag was expressed in HEK293 cells. |
| Molecular weight | Recombinant CD25 is a monomer protein consisting of 203 amino acid residue subunits, due to glycosylation migrates as an approximately44 kDa protein on SDS-PAGE. |
| Purity | >97%, as determined by SDS-PAGE and HPLC |
| Biological Activity | The activity of recombinant IL-2Ra (CD25) was determined by its ability to bind Human IL-2 with an affinity constant of 30nM . |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
| Presentation | Recombinant CD25 is supplied as a 0.2 μm filtered PBS solution, pH7.4 . |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to prepare a stock solution concentration not less than 0.1 mg/mL. |
| Storage | Recombinant CD25, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles. |
| Usage | This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | P14784 IL2RB_HUMAN |
| Biological Process | Immunity |
| Molecular function | Receptor |
Methods
sIL-2Rα treated mice
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