Human IL-7 receptor Recombinant (CD127)

//Human IL-7 receptor Recombinant (CD127)

Human IL-7 receptor Recombinant (CD127)

$160.00$1,950.00


accession P16871


Source Optimized DNA sequence encoding extracellular domain of Human IL-7 receptor alpha including a C-terminal His tag was expressed in HEK293 cells.
Molecular weight Recombinant Human IL-7 receptor alpha (CD127) is a protein consisting of 226 amino acid residue subunits, due to glycosylation migrates as an approximately45 kDa protein on SDS-PAGE.
Purity >97%, as determined by SDS-PAGE and HPLC
Biological Activity Recombinant Human IL-7 receptor alpha (CD127) Activity was determined by its binding ability of biotinylated mouse TSLP in a functional ELISA.
Presentation Recombinant Human IL-7R (CD127) is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Storage Recombinant Human IL-7 receptor can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor P13232 IL7_HUMAN
Molecular function Receptor

Methods

Bioinformatic analysis for prediction of sCD127 glycation sites and experimental glycation of sCD127.

  • ecombinant human sC127 was experimentally glycated by incubation for 7 days in the dark at 37°C in the presence of 100 mmol/L d-glucose (Sigma-Aldrich) or PBS as control.
  • sCD127 glycation was evaluated by nontryptophan fluorescence as previously reported (