Human IL-28B Recombinant

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Human IL-28B Recombinant


accession Q8IZI9

Source DNA sequence encoding mature chain of Human IL28B to a C-terminal polyHis tag was expressed in CHO cells.
Molecular weight Recombinant Human Interleukin-28B (Interferon lambda) is a monomer consisting of 186 amino acid residue , and migrates as an approximately23 kDa protein under reducing conditions in SDS-PAGE.
Purity >97%, as determined by SDS-PAGE
Biological Activity The activity was determined by a WISH cell viral resistance assay with a linear range of 4-20 ng/ml .
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human Interleukin-28B was lyophilized from a 0.2 μm filtered PBS solution pH 7.4.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Biological Process Antiviral-defense
Molecular function Cytokine


pDC-GEN2.2 cells sense HCV PAMP and produce Type I and Type III IFNs.

  • H) Western Blot of pDC-GEN2.2 cell lysates for IL-28B/IFNλ3 after 24 hours of HCV PAMP stimulation.