Human IL-27 Recombinant

Human IL-27 Recombinant

$480.00$3,700.00


accession Q8NEV9


Source DNA sequence encodingmature chain of Human IL-27A fused toDNA sequence encodingmature chain of Human IL-27B via a linker chain anda C-terminal polyHis tag was expressed in CHO cells.
Molecular weight Recombinant Human Interleukin-27 is a monomer consisting of 450 amino acid residue subunits (215 subunit A + 209 subunit B +linker), and migrates as an approximately50 kDa protein under reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE
Biological Activity The activity was determined by its ability to bind recombinant Human IL-27RA with a linear range of 60-1000 ng/ml in functional ELISA .
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human Interleukin-27 was lyophilized from a 0.2 μm filtered PBS solution pH 7.4.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor Q14213 IL27B_HUMAN
Biological Process Immunity
Biological Process Inflammatory-response
Molecular function Antiviral-protein
Molecular function Cytokine