Human IL-12B p40 Recombinant

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Human IL-12B p40 Recombinant


accession P29460

Source DNA sequence encoding extracellular domain of Human IL-12B fused to a C-terminal polyHis tag wasexpressed in HEK cells.
Molecular weight Recombinant Human Interleukin-12B p40 subunit is a monomer consisting of 306 amino acid residue , and migrates as an approximately46 kDa protein under reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE
Biological Activity The activity was determined by its ability to bind recombinant Human IL-12RB1 in functional ELISA .
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human IL-12B p40 was lyophilized from a 0.2 μm filtered PBS solution pH 7.4.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Interactor P29459 IL12A_HUMAN
Interactor P01730 CD4_HUMAN
Molecular function Cytokine