Human IGF-I Recombinant

Human IGF-I Recombinant

$70.00$270.00


accession P01343


Source Optimized DNA sequence encoding Human Insulin Growth Factor -1mature chain was expressed in Escherichia Coli.
Molecular weight Native Human Insulin Growth Factor-1 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately kDa. Recombinant Human IGF-1 is a monomeric protein consisting of amino acid residue subunits. IGF-1 migrates as an approximately kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity The ED50 was determined by a cell proliferation assay using FDC-P1 cells is ≤.0 ng/ml, corresponding to a specific activity of ≥1 x units/mg.
Protein Sequence MGKISSLPTQ LFKCCFCDFL KVKMHTMSSS HLFYLALCLL TFTSSATAGP ETLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSA RS VRAQRHTDMP KTQKEVHLKN ASRGSAGNKN YRM
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant Human IGF1was lyophilized from a.2 μm filtered PBS solution, pH.4.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.



Methods

Effect of vorinostat on IGF-I-mediated signal transduction.

IGF-I
  • Ishikawa and USPC-2 cells were treated for 24 h with vorinostat (or left untreated) and/or IGF-I during the last 10 min of the incubation period.

Induction of lamellipodia formation and invasion by IGF-I in MDA-MB-231 cells but not MCF7 cells.

50 ng/mL IGF-I
  • (a) After stimulation with or without (mock) IGF-I, cells were stained with phalloidin.

Cell preparation

  • Human ESC-CMs and non-cardiac hESC derivatives were harvested and cryopreserved after 16–18 days under differentiating conditions.
  • One day prior to harvest, cells were subjected to a pro-survival protocol, previously shown to enhance engraftment post-transplantation.
  • In brief, cultures were heat-shocked with a 30-minute exposure to 43 °C medium, followed by RPMI-B27 medium supplemented with IGF-1 (100 ng/ml) and cyclosporine A (0.2 μM, , ).
  • One day later, cultures were harvested with 0.25% trypsin/0.5 mM EDTA and cryopreserved as previously described.

Materials

  • Hank's Balanced Sodium Salt (HBSS), medium'>Dulbecco's medium'>modified medium'>Eagle's medium (DMEM) high glucose (4.5 g/L), alpha minimum essential medium, phosphate-buffered salt (PBS), penicillin/streptomycin, trypsin/EDTA (0.05%/0.53 mM), L-glutamine, superscript III kit, NuPAGE 4%–12% Bis–Tris gel, bone morphogenetic protein-2 (BMP-2), and polyvinylidene difluoride (PVDF)membranes were obtained from .
  • Low-molecular weight heparin (4,500 g/mol) was obtained from (, http://www.sanofi.com/).
  • 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) reagents were from (, http://www.promega.com/products).
  • Total collagens and GAG quantification kits were from (, http://www.biocolor.co.uk).
  • Brilliant SYBR Green Master Mix was obtained from ( , The http://www.stratagene.com).
  • PCR primers were synthesized by MWG (, http://www.mwg-biotech.com).
  • TGF-β1 and Insulin-like growth factor-1 (IGF-1) were obtained from (, http://www.peprotechec.com).
  • Protein content was determined using the assay (, , http://www.piercenet.com).
  • The Western blot detection system was obtained from GE Healthcare (Buckin-ghamshire, UK, http://www3.gehealthcare.com).
  • All other chemicals were obtained from standard laboratory suppliers and were of the highest purity…

Effects of IGF-1-containing gelatin hydrogel on neurogenesis in the SVZ.

recombinant human IGF-1
  • (b) Coronal sections of brains that received an injection of microspheres containing IGF-1 or microspheres plus PBS, showing DCX+ new neurons in the SVZ (green) (n = 5 animals for each group).

Effects of Activin A on paraxial mesodermal differentiation of mouse iPS cells.

10 ng/ml recombinant human IGF-1
  • The cultures also contained BMP4 (10 ng/ml), IGF-1 (10 ng/ml), LiCl (5 mM), and Shh (10 ng/ml).

In vitro Embryo Cultures

  • Tri-pronuclear zygotes were cultured individually in 30 µl microdrops containing the Global-Medium with 5% human serum albumin in the presence or absence of EGF, IGF-I, GM-CSF, BDNF, and CSF-1 , all at 10 ng/ml.
  • The culture medium was renewed every 48 h. Embryonic development was evaluated at 96, 120, and 144 h after culture.

In vitro Embryo Cultures

  • Normally fertilized embryos frozen on day 3 of culture by slow cooling were thawed by using a 2-step thawing protocol 2, 5% O2 and 90% N2 with or without growth factor mixtures containing 10 ng/ml of EGF, IGF-I, GM-CSF, BNF, CSF-1, artemin, and GNF .
  • Individual embryos were cultured for 72 h in a 30 µl drop of medium and their development was evaluated.
  • The doses of these growth factors chosen for these experiments were based on previous studies (BDNF

Cell cycle analysis of MCF-7 cells in the presence of IGF-1 at 8 h. MCF-7 cells were serum starved for 24 h. At 22 h after serum starvation the cells were treated with 10 μM auraptene in 0.01% DMSO.

Recombinant human IGF-1
  • At 24 h serum starvation, the cells were treated with IGF-1 (10 ng/mL).

Effect of metformin on IGF-I-mediated signal transduction and mTOR and Ampk signalling pathway in endometrial cancer cells.

IGF-I
  • A, Ishikawa, ECC-1, USPC-2 and USPC-1 cells were treated with metformin (10 mM) for 24 h (or left untreated) in the presence or absence of IGF-I (50 ng/ml) during the last 10 min of the incubation period.