Human Hepatocyte Growth Factor Recombinant
Categories: PDGF familyPDGF familyRecombinant Human Cytokines$70.00 – $4,300.00
Description
Accession
P14210
Source
Optimized DNA sequence encoding Human Hepatocyte Growth Factor mature chain was expressed in Chinese Hamster Ovary cell line.
Molecular weight
Native human Hepatocyte Growth Factor is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately kDa. Recombinant HGF is a glycosylated disulfide-linked heterodimeric protein consisting of alpha chain (463)and the beta chain (234)amino acid residue subunits, and migrates as an approximately 80 kDa protein under non reducing conditions in SDS-PAGE.
Purity
>97%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by the dose-dependent stimulation of the proliferation ofmurine BNL-CL2cell li was found to be in the range of.0-40.0 ng/ml.
Protein Sequence
MWVTKLLPAL LLQHVLLHLL LLPIAIPYAE GQRKRRNTIH EFKKSAKTTL IKIDPALKIK TKKVNTADQC ANRCTRNKGL PFTCKAFVFD KARKQCLWFP FNSMSSGVKK EFGHEFDLYE NKDYIRNCII GKGRSYKGTV SITKSGIKCQ PWSSMIPHEH SFLPSSYRGK DLQENYCRNP RGEEGGPWCF TSNPEVRYEV CDIPQCSEVE CMTCNGESYR GLMDHTESGK ICQRWDHQTP HRHKFLPERY PDKGFDDNYC RNPDGQPRPW CYTLDPHTRW EYCAIKTCAD NTMNDTDVPL ETTECIQGQG EGYRGTVNTI WNGIPCQRWD SQYPHEHDMT PENFKCKDLR ENYCRNPDGS ESPWCFTTDP NIRVGYCSQI PNCDMSHGQD CYRGNGKNYM GNLSQTRSGL TCSMWDKNME DLHRHIFWEP DASKLNENYC RNPDDDAHGP WCYTGNPLIP WDYCPISRCE GDTTPTIVNL DHPVISCAKT KQLRVVNGIP TRTNIGWMVS LRYRNKHICG GSLIKESWVL TARQCFPSRD LKDYEAWLGI HDVHGRGDEK CKQVLNVSQL VYGPEGSDLV LMKLARPAVL DDFVSTIDLP NYGCTIPEKT SCSVYGWGYT GLINYDGLLR VAHLYIMGNE KCSQHHRGKV TLNESEICAG AEKIGSGPCE GDYGGPLVCE QHKMRMVLGV IVPGRGCAIP NRPGIFVRVA YYAKWIHKII LTYKVPQS
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Interleukin-6 was lyophilized from a 0.2 μm filtered solution in.5% glycine,.5% sucrose,.01% Tween80, mM Glutamic acid, pH.5.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
Molecular function
Molecular function
Methods
Differentiation from human iPS cells toward hepatic lineage cells.
- Human iPS cells were sequentially stimulated with various cytokines: (1) activin A, (2) basic FGF and BMP-4, and (3) HGF.
Differentiation from human iPS cells toward hepatic lineage cells.
- Human iPS cells were sequentially stimulated with various cytokines: (1) activin A, (2) basic FGF and BMP-4, and (3) HGF.
Differentiation of NPCs into renal epithelial cells consisting of nephrons
- hPSC-derived NPCs were re-plated on fibronectin-coated dishes and stabilized in the same differentiation medium containing BMP7 (150 ng/ml) and FGF2 (50 ng/ml) for one day.
- They were incubated in renal epithelial cell growth medium (REGMâ„¢;, , ) supplemented with 50 ng/ml HGF to develop into renal tubular epithelial cells for 21 days.
- For differentiation into glomerular podocytes, NPCs were cultured in VRAD medium, which consisted of DMEM/F12, 100 nM Vitamin D3 , 100 µM RA and 10% FBS for seven days as previously described
Vinculin is specifically tyrosine phosphorylated at Y822 in cell–cell junctions.
- The levels of phosphorylated and total vinculin in MCF10a cells induced to scatter by application of HGF for 2 h or in MDCK cells overexpressing Snail were examined and presented as described in A.
Hepatic differentiation protocols
- To induce hepatogenic differentiation, AT-MSCs were plated after three passages on 5-mm culture dishes coated with collagen type I in expansion medium (DMEM-LG supplemented with 10% FBS).
- Following reaching confluence, cells were washed twice with PBS and cultured in basic hepatic differentiation medium supplemented with 1X insulin-transferrin-selenium (ITS), 10−8 M dexamethasone , 20 ng/ml epidermal growth factor , 20 ng/ml fibroblast growth factor (FGF ), 40 ng/ml oncostatin M (OSM) and 40 ng/ml hepatocyte growth factor (HGF) .
- After 2 weeks, the medium was replaced with hepatic differentiation medium with an increased concentration of dexamethasone at 10−5 M (
Cell culture
-
Immortalized human myoblasts were obtained from the University of Massachusetts Medical School Senator Paul D. Wellstone Muscular Dystrophy Cooperative Research Center for FSHD (
http://www.umassmed.edu/wellstone ) in, , . - The immortalized control myoblasts (WS161, 01Ubic CT#5) were derived from the biceps of a healthy 46-year-old male (
l -glutamine; Euroclone) (Gibco) supplemented with 15% fetal bovine serum (FBS) (Gibco), 0.02m Hydroxyethyl-Piperazine Ethanesulafonic Acid (HEPES), pH 7.2, 0.03 μg/ml ZnSO4, 1.4 μg/ml vitamin B12 (Sigma-Aldrich), 0.055 μg/ml dexamethasone (Sigma-Aldrich), 1% penicillin/streptomycin (100 U/ml final concentration), 2.5 ng/ml hepatocyte growth factor (PeproTech) and 10 ng/ml basic fibroblast growth factor (PeproTech)]. - When 90% confluent, cells were switched to differentiation medium [4:1 DMEM:Medium 199 supplemented with 2% horse serum and 1% penicillin/streptomycin] for 4 days.
- Medium was replaced with fresh differentiation medium every day.
- Muscle cells were routinely cultured in a humidified atmosphere at 37°C with 5% O2 and 5% CO2.
- The culture dishes were coated…
