Human HCC-1(CCL14) Recombinant, biotinylated

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Human HCC-1(CCL14) Recombinant, biotinylated


accession Q16627

Source DNA sequence encoding Human CCL-14 mature chain was expressed in Escherichia Coli. CCL14 was further labeled by Biotin for detection by Avidin conjugated secondary probes
Molecular weight Human HCC-1, generated by the proteolytic removal of the signal peptide and propeptide and has a calculated molecular mass of approximately 9 kDa. Recombinant HCC-1 is a monomeric protein consisting of 72 amino acid residue subunits and migrates as an approximately9 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Purity >98%, as determined by SDS-PAGE and HPLC
Applications Biotinylated CCL14 was designed to be used in single step detection in ELISA ,WB
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant HCC-1 was lyophilized from a.2 μm filteredmM PB,100mM NaCl solution pH.5.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Interactor P32246
Molecular function Cytokine