Human GRO-alpha (CXCL1)Recombinant
$70.00 – $2,700.00
| accession | P09341 |
| Source | Optimized DNA sequence encoding Human GRO alpha mature chain was expressed in Escherichia Coli. |
| Molecular weight | Native human GRO-alpha is generated by the proteolytic removal of the signal peptide and propeptide. The molecule has a calculated molecular mass of approximately 8 kDa. Recombinant GRO-a is a monomer protein consisting of 73 amino acid residue subunits, andmigrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE. |
| Purity | >97%, as determined by SDS-PAGE and HPLC |
| Biological Activity | Determined by theability to chemoattract human neutrophils using a concentration range of.0-50.0 ng/ml. |
| Protein Sequence | MARAALSAAP SNPRLLRVAL LLLLLVAAGR RAAGASVATE LRCQCLQTLQ GIHPKNIQSV NVKSPGPHCA QTEVIATLKN GRKACLNPAS PIVKKIIEKM LNSDKSN |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
| Presentation | Recombinant GRO-alpha/CXCL1was lyophilized from a.2μm filtered concentrated (1mg/ml) solution inmM NaCl,mM PB, pH7.0. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | P25025 |
| Interactor | Q16570 |
| Interactor | P25024 |
| Biological Process | Inflammatory-response |
| Molecular function | Cytokine |
| Molecular function | Growth-factor |
Methods
Immunofluorescence Confocal microscopy
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