Human GM-CSF Recombinant (CHO cell)

////Human GM-CSF Recombinant (CHO cell)

Human GM-CSF Recombinant (CHO cell)

$160.00$3,500.00


accession P04141


Source Optimized DNA sequence encoding Human Granulocyte Macrophage-Colony Stimulating Factor mature chain was expressed in CHO Cells.
Molecular weight Native human Granulocyte Macrophage-Colony Stimulating Factor is generated by the proteolytic removal of the signal peptide and propeptide,this molecule has a calculated mass of kDa. Recombinant GM-CSFis a monomer protein, consisting of amino acidsand due to glycosylation migrates as an approximately kDa protein under reducing conditions.
Purity >96%, as determined by SDS-PAGE and HPLC
Biological Activity The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤.1 ng/ml, corresponding to a specific activity of ≥ x units/mg.
Protein Sequence MWLQSLLLLG TVACSISAPA RSPSPSTQPW EHVNAIQEAR RLLNLSRDTA AEMNETVEVI SEMFDLQEPT CLQTRLELYK QGLRGSLTKL KGPLTMMASH YKQHCPPTPE TSCATQIITF ESFKENLKDF LLVIPFDCWE PVQE
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Human GM-CSFwas lyophilized from a.2 μm filtered solution in PBS, pH7.0.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor P15509 CSF2R_HUMAN
Interactor Q99062 CSF3R_HUMAN
Interactor P32927 IL3RB_HUMAN
Molecular function Cytokine
Molecular function Growth-factor