Human Glia Maturation Factor gamma Recombinant
Categories: Neurotrophic factorsRecombinant Human Cytokines$70.00 – $4,700.00
Description
Accession
O60234
Source
Optimized DNA sequence encoding Human Glia Maturation Factor gamma(GMFG) mature chain was expressed in Escherichia Coli.
Molecular weight
Native human Glia Maturation Factor gamma, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 19kDa. Recombinant Glia Maturation Factor is a monomer protein consisting of 160 amino acid residue subunits,and mi9rates as an approximately 17kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity
>98%, as determined by SDS-PAGE and HPLC
Biological Activity
ED50 not determined.
Protein Sequence
MSDSLVVCEV DPELTEKLRK FRFRKETDNA AIIMKVDKDR QMVVLEEEFQ NISPEELKME LPERQPRFVV YSYKYVHDDG RVSYPLCFIF SSPVGCKPEQ QMMYAGSKNR LVQTAELTKV FEIRTTDDLT EAWLQEKLSF FR
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant GMFG was lyophilized from a 0.2 μm filteredmM Tris-HCl (pH8.0) containingmM DTT,mM EDTA% glycerol.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
