Human G-CSF Recombinant (CHO cell)
$160.00 – $4,700.00
| accession | P09919 |
| Source | Optimized DNA sequence encoding Human G-CSF mature chain was expressed in CHO Cells |
| Molecular weight | Native human G-CSF is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 19 kDa. Recombinant GCSF is a protein consisting of 175 amino acid residue subunits, due to glycosylation migrates as an approximately 22 kDa protein under reducing conditions in SDS-PAGE. |
| Purity | >98%, as determined by SDS-PAGE and HPLC |
| Biological Activity | The ED(50) was determined by the dose-dependent proliferation of murine M-NFS-60 cells is ≤.1 ng/ml, corresponding to a specific activity of ≥ xunits/mg. |
| Protein Sequence | MAGPATQSPM KLMALQLLLW HSALWTVQEA TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCA TYKLCHPEEL VLLGHSLGIP WAPLSSCPSQ ALQLAGCLSQ LHSGLFLYQG LLQALEGISP ELGPTLDTLQ LDVADFATTI WQQMEELGMA PALQPTQGAM PAFASAFQRR AGGVLVASHL QSFLEVSYRV LRHLAQP |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
| Presentation | Recombinant G-CSF was lyophilized from a.2 μm filtered PBS solution pH7.0. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | P40223 |
| Molecular function | Cytokine |
| Molecular function | Growth-factor |