| accession | Q9GZV9 |
| Source | Optimized DNA sequence encoding Human Fibroblast Growth Factor-23 mature chain was expressed in Escherichia Coli. |
| Molecular weight | Native human FGF23 is generated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated molecular mass of approximately23 kDa. Recombinant Fibroblast Growth Factor is a disulfide-linked monomer protein consisting of amino acid residue subunits,and migrates as an approximately23 kDa protein under non-reducing andreducing conditions in SDS-PAGE. |
| Purity | >96%, as determined by SDS-PAGE and HPLC |
| Biological Activity | The ED(50) was determined by the dose-dependent proliferation of mouseT3 cells expressing FGF receptors, and was found to be <0.3ug/ml., corresponding to a specific activity of.0 x Units/mg. |
| Protein Sequence | MLGARLRLWV CALCSVCSMS VLRAYPNASP LLGSSWGGLI HLYTATARNS YHLQIHKNGH VDGAPHQTIY SALMIRSEDA GFVVITGVMS RRYLCMDFRG NIFGSHYFDP ENCRFQHQTL ENGYDVYHSP QYHFLVSLGR AKRAFLPGMN PPPYSQFLSR RNEIPLIHFN TPIPRRHTRS AEDDSERDPL NVLKPRARMT PAPASCSQEL PSAEDNSPMA SDPLGVVRGG RVNTHAGGTG PEGCRPFAKF I |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
| Presentation | Recombinant Human FGF23 was lyophilized from.2 μm filtered PBS solution, pH.4 . |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Biological Process | Differentiation |
| Molecular function | Growth-factor |
Human Fibroblast Growth Factor 23 Recombinant
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