Human Fibroblast Growth Factor 10 Recombinant Biotinylated

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Human Fibroblast Growth Factor 10 Recombinant Biotinylated

$480.00$4,800.00

SKU: RKO15520B Categories: , , , Tags: , ,

accession O15520


Source DNA sequence encoding Human Fibroblast Growth Factor-10 mature chain was expressed in Escherichia Coli.
Molecular weight Native human FGF10 is generated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated molecular mass of approximately 19 kDa. Recombinant Fibroblast Growth Factor is a monomer protein consisting of 170 amino acid residue subunits,and migrates as an approximately 19 kDa protein reducing conditions in SDS-PAGE.
Purity >96%, as determined by SDS-PAGE and HPLC
Biological Activity The ED(50) was determined by the dose-dependent proliferation of BaF3 cells expressing FGF receptors, and was found to be <0.5ng/ml.

, corresponding to a specific activity of 2.0 x10^7 Units/mg.
Protein Sequence QAL GQDMVSPEAT NSSSSSFSSP SSAGRHVRSY NHLQGDVRWR KLFSFTKYFL KIEKNGKVSG TKKENCPYSI LEITSVEIGV VAVKAINSNY YLAMNKKGKL YGSKEFNNDC KLKERIEENG YNTYASFNWQ HNGRQMYVAL NGKGAPRRGQ KTRRKNTSAH FLPMVVHS
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation RecombinantFGF10 was lyophilized from.2 μm filtered PBS solution, pH7.0 .
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor P21802 FGFR2_HUMAN
Interactor P21803 FGFR2_MOUSE
Molecular function Growth-factor