Human Exodus-2 Recombinant

///Human Exodus-2 Recombinant

Human Exodus-2 Recombinant

$70.00$2,700.00

SKU: RKO00585 Category: Tags: , , , , ,

accession O00585


Source Optimized DNA sequence encoding HumanExodus-2 mature chain was expressed in Escherichia Coli.
Molecular weight Native human Exodus-2 isgenerated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated molecular mass of approximately12 kDa. Recombinant Exodus-2 is a disulfide-linked homodimeric protein consisting of112 amino acid residue subunits, andmigrates as an approximately12 kDa protein under non-reducingand reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity Determined by its ability to chemoattract total lymphocyte population using a concentration range of.0-100.0 ng/ml.

Protein Sequence MAQSLALSLL ILVLAFGIPR TQGSDGGAQD CCLKYSQRKI PAKVVRSYRK QEPSLGCSIP AILFLPRKRS QAELCADPKE LWVQQLMQHL DKTPSPQKPA QGCRKDRGAS KTGKKGKGSK GCKRTERSQT PKGP
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant Exodus-2 was lyophilized from a.2 μm filtered solution in.5% glycine,.5% sucrose,.01% Tween80, mM Glutamic acid, pH.5.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor O43927 CXL13_HUMAN
Interactor P32248
Biological Process Chemotaxis
Biological Process Inflammatory-response
Molecular function Cytokine

Methods

Effect of ADA on CCR7 expression and iDC migration.

CCL21 chemokines
  • The percentage of cell migration to CCL19 or CCL21 in the absence or in the presence of 2 µM ADA or in the presence of maturating cocktail (mDCs), in relation to the initial cell input is shown.

Phenotype and migration of DC matured in IRX-2 or conventional cytokines.

CCL21
  • While iDC showed very little migration in response to CCL21, both conventional- and IRX-2-matured DC migrated significantly better.