Description
Accession
O00585
Source
Optimized DNA sequence encoding Human Exodus-2 CCL21 mature chain was expressed in Escherichia Coli.
Molecular weight
Native human Exodus-2 CCL21 is generated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated molecular mass of approximately 12 kDa. Recombinant Exodus-2 is a monomer protein consisting of 112 amino acid residue subunits, and migrates as an approximately 12 kDa protein under reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
Determined by its ability to chemoattract total lymphocyte population using a concentration range of.0-100.0 ng/ml.
Protein Sequence
MAQSLALSLL ILVLAFGIPR TQGSDGGAQD CCLKYSQRKI PAKVVRSYRK QEPSLGCSIP AILFLPRKRS QAELCADPKE LWVQQLMQHL DKTPSPQKPA QGCRKDRGAS KTGKKGKGSK GCKRTERSQT PKGP
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Exodus-2 was lyophilized from a 0.2 μm filtered solution in.5% glycine,.5% sucrose,.01% Tween80, mM Glutamic acid, pH.5.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
P32248
Biological Process
Biological Process
Molecular function
Methods
Effect of ADA on CCR7 expression and iDC migration.
- The percentage of cell migration to CCL19 or CCL21 in the absence or in the presence of 2 µM ADA or in the presence of maturating cocktail (mDCs), in relation to the initial cell input is shown.
Phenotype and migration of DC matured in IRX-2 or conventional cytokines.
- While iDC showed very little migration in response to CCL21, both conventional- and IRX-2-matured DC migrated significantly better.
