Human Exodus-2 Recombinant
$70.00 – $2,700.00
| accession | O00585 |
| Source | Optimized DNA sequence encoding Human Exodus-2 CCL21 mature chain was expressed in Escherichia Coli. |
| Molecular weight | Native human Exodus-2 CCL21 is generated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated molecular mass of approximately 12 kDa. Recombinant Exodus-2 is a monomer protein consisting of 112 amino acid residue subunits, and migrates as an approximately 12 kDa protein under reducing conditions in SDS-PAGE. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Biological Activity | Determined by its ability to chemoattract total lymphocyte population using a concentration range of.0-100.0 ng/ml. |
| Protein Sequence | MAQSLALSLL ILVLAFGIPR TQGSDGGAQD CCLKYSQRKI PAKVVRSYRK QEPSLGCSIP AILFLPRKRS QAELCADPKE LWVQQLMQHL DKTPSPQKPA QGCRKDRGAS KTGKKGKGSK GCKRTERSQT PKGP |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
| Presentation | Recombinant Exodus-2 was lyophilized from a.2 μm filtered solution in.5% glycine,.5% sucrose,.01% Tween80, mM Glutamic acid, pH.5. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | O43927 CXL13_HUMAN |
| Interactor | P32248 |
| Biological Process | Chemotaxis |
| Biological Process | Inflammatory-response |
| Molecular function | Cytokine |
Methods
Effect of ADA on CCR7 expression and iDC migration.
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Phenotype and migration of DC matured in IRX-2 or conventional cytokines.
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