Human Epidermal Growth Factor Recombinant , plant derived

/, EGF family, Recombinant Human Cytokines/Human Epidermal Growth Factor Recombinant , plant derived

Human Epidermal Growth Factor Recombinant , plant derived


accession P01133

Source DNA sequence encoding Human EGF mature chain was expressed in cereal grain.
Molecular weight Recombinant Human EGF contains 53 amino acids and migrates as an approximately 6 kDa protein under reducing conditions in SDS-PAGE
Purity >98%, as determined by SDS-PAGE and HPLC
Biological Activity The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥2 x 107 units/mg.
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation RecombinantEpidermal Growth Factor was lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in PBS, pH 7.2.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Interactor P00533 EGFR_HUMAN
Molecular function Growth-factor