Human Epidermal Growth Factor Recombinant , plant derived
$600.00
| accession | P01133 |
| Source | DNA sequence encoding Human EGF mature chain was expressed in cereal grain. |
| Molecular weight | Recombinant Human EGF contains 53 amino acids and migrates as an approximately 6 kDa protein under reducing conditions in SDS-PAGE |
| Purity | >98%, as determined by SDS-PAGE and HPLC |
| Biological Activity | The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥2 x 107 units/mg. |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
| Presentation | RecombinantEpidermal Growth Factor was lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in PBS, pH 7.2. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | P00533 EGFR_HUMAN |
| Molecular function | Growth-factor |