Human EpCAM Recombinant, Biotinylated
Categories: Biotinylated proteinsSurface & Soluble Receptors$340.00 – $1,950.00
Description
Accession
P16422
Source
Optimized DNA sequence encoding extracellular domain of Human Epithelial cell adhesion molecule (EpCAM)(GLN24 - LYS265)including a C-terminal His tag was expressed in HEK293 cells. Recombinant Human EpCAM was further labeled by Biotin for detection by Avidin conjugated secondary probes.
Molecular weight
Recombinant human EpCAM is a protein consisting of 249 amino acid residue subunits,due to glycosylation migrates as an approximately 35kDa protein on reduced SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Applications
ELISA,WB Biotinylated recombinant EpCAM was designed to be used in single step detection in ELISA ,WB.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human EpCAM is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Storage
Recombinant EpCAM can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
