Description
Accession
O00175
Source
Optimized DNA sequence encoding HumanEotaxin-2 mature chain was expressed in Escherichia Coli.
Molecular weight
Native human Eotaxin-2 has a calculated molecular mass of approximately 9 kDa. Recombinant Eotaxin-2 is a monomer protein consisting of 79 amino acid residue subunits, and migrates as an approximately 9 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity
>97%, as determined by SDS-PAGE and HPLC
Biological Activity
Activity was determined by the ability to induce chemotaxis of purified eosinophils at concentrations ranging between-100 ng/ml.
Protein Sequence
MAGLMTIVTS LLFLGVCAHH IIPTGSVVIP SPCCMFFVSK RIPENRVVSY QLSSRSTCLK AGVIFTTKKG QQFCGDPKQE WVQRYMKNLD AKQKKASPRA RAVA VKGPVQ RYPGNQTTC
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Eotaxin-2 waslyophilzed from.2μm filtered concentrated (1.0mg/ml) solution inmM NaCl,mM PB, pH7.0.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
P51677
Biological Process
Biological Process
Molecular function
Methods
SPR sensorgrams for binding of hCCR3 with its ligands.
- The sensorgrams show the binding of hCCR3 with CCL11 and CCL24 in the presence of FC-14 and the binding of hCCR3 with CCL11 and CCL24 in the presence of DDM.
