Human Eotaxin-1 (CCL11 ) Recombinant

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Human Eotaxin-1 (CCL11 ) Recombinant

$70.00$2,700.00


accession P51671


Source Optimized DNA sequence encoding HumanEotaxin-1 mature chain was expressed in Escherichia Coli.
Molecular weight Native human Eotaxin-1 has a calculated molecular mass of approximately9 kDa. Recombinant Eotaxin-1 is a disulfide-linked homodimeric protein consisting of amino acid residue subunits, andmigrates as an approximately9 kDa protein under non-reducingand reducing conditions in SDS-PAGE.
Purity >97%, as determined by SDS-PAGE and HPLC
Biological Activity Activitywas determined by the ability to induce chemotaxis of purified eosinophils at concentrations ranging between-100 ng/ml.

Protein Sequence MKVSAALLWL LLIAAAFSPQ GLAGPASVPT TCCFNLANRK IPLQRLESYR RITSGKCPQK AVIFKTKLAK DICADPKKKW VQDSMKYLDQ KSPTPKP
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant Eotaxin-1 wasis lyophilized from as.2μm filtered concentrated (1.0mg/ml) PBS solution, pH.4.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor P49682
Interactor P51677
Interactor P51681
Biological Process Chemotaxis
Biological Process Inflammatory-response
Molecular function Cytokine

Methods

SPR sensorgrams for binding of hCCR3 with its ligands.

The commercial recombinant human chemokines CCL11
  • The sensorgrams show the binding of hCCR3 with CCL11 and CCL24 in the presence of FC-14 and the binding of hCCR3 with CCL11 and CCL24 in the presence of DDM.