Human Eotaxin-1 (CCL11 ) Recombinant

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Human Eotaxin-1 (CCL11 ) Recombinant

$70.00$2,700.00


accession P51671


Source Optimized DNA sequence encoding HumanEotaxin-1 mature chain was expressed in Escherichia Coli.
Molecular weight Native human Eotaxin-1 has a calculated molecular mass of approximately9 kDa. Recombinant Eotaxin-1 is a disulfide-linked homodimeric protein consisting of amino acid residue subunits, andmigrates as an approximately9 kDa protein under non-reducingand reducing conditions in SDS-PAGE.
Purity >97%, as determined by SDS-PAGE and HPLC
Biological Activity Activitywas determined by the ability to induce chemotaxis of purified eosinophils at concentrations ranging between-100 ng/ml.

Protein Sequence MKVSAALLWL LLIAAAFSPQ GLAGPASVPT TCCFNLANRK IPLQRLESYR RITSGKCPQK AVIFKTKLAK DICADPKKKW VQDSMKYLDQ KSPTPKP
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant Eotaxin-1 wasis lyophilized from as.2μm filtered concentrated (1.0mg/ml) PBS solution, pH.4.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Biological Process Chemotaxis
Biological Process Inflammatory-response
Molecular function Cytokine

Methods

SPR sensorgrams for binding of hCCR3 with its ligands.

The commercial recombinant human chemokines CCL11
  • The sensorgrams show the binding of hCCR3 with CCL11 and CCL24 in the presence of FC-14 and the binding of hCCR3 with CCL11 and CCL24 in the presence of DDM.