Description
Accession
P39060
Source
Optimized DNA sequence encoding HumanEndostatin mature chain was expressed in Escherichia Coli.
Molecular weight
Native human Endostatin is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 20 kDa. Recombinant Endostatinis a monomer protein consisting of 184 amino acid residue subunits, and migrates as an approximately 20 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity
>98%, as determined by SDS-PAGE and HPLC
Biological Activity
Protein Sequence
1570 1580 1590 1600 1610 1620 LRPARPTSPP AHSHRDFQPV LHLVALNSPL SGGMRGIRGA DFQCFQQARA VGLAGTFRAF 1630 1640 1650 1660 1670 1680 LSSRLQDLYS IVRRADRAAV PIVNLKDELL FPSWEALFSG SEGPLKPGAR IFSFDGKDVL 1690 1700 1710 1720 1730 1740 RHPTWPQKSV WHGSDPNGRR LTESYCETWR TEAPSATGQA SSLLGGRLLG QSAASCHHAY 1750 IVLCIENSFM TASK
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Endostatinwas lyophilized from a 0.2 μm filtered PBS solution pH 7.5.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
