Human EMAP-II Recombinant

Human EMAP-II Recombinant

$70.00$3,500.00

SKU: RKQ12904 Category: Tags: , , , , ,

accession Q12904


Source Optimized DNA sequence encoding Human EMAP-II mature chain was expressed in Escherichia Coli.
Molecular weight Nativehuman EMAP-II, generated by the proteolytic removal by caspase-7 in response to apoptosis to produce EMAP-II, the molecule has a calculated molecular mass of approximately18kDa. Recombinant EMAP-II is a monomer protein consisting of amino acid residue subunits,and migrates as an approximately18kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity The ED(50) was determined by apoptotic effect on MCF-7 cells using a concentration of-50 ng/ml.

Protein Sequence MANNDAVLKR LEQKGAEADQ IIEYLKQQVS LLKEKAILQA TLREEKKLRV ENAKLKKEIE ELKQELIQAE IQNGVKQIPF PSGTPLHANS MVSENVIQST AVTTVSSGTK EQIKGGTGDE KKAKEKIEKK GEKKEKKQQS IAGSADSKPI DVSRLDLRIG CIITARKHPD ADSLYVEEVD VGEIAPRTVV SGLVNHVPLE QMQNRMVILL CNLKPAKMRG VLSQAMVMCA SSPEKIEILA PPNGSVPGDR ITFDAFPGEP DKELNPKKKI WEQIQPDLHT NDECVATYKG VPFEVKGKGV CRAQTMSNSG IK
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant EMAP-II was lyophilized from a.2 μm filteredmM PB,130mM NaCl solution pH.5.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Biological Process Angiogenesis
Biological Process Carbohydrate-metabolism
Biological Process Cell-adhesion
Molecular function Cytokine
Molecular function RNA-binding